 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 6 | 1/1 | 返回列表 |
| 查看: 679 | 回復(fù): 5 | ||
金獅水友至尊木蟲 (著名寫手)
|
[求助]
英譯中---生物傳感器論文--求科學(xué)的翻譯
|
|
Apart from its high sensitivity, as compared to other electrochemical miRNA biosensors, the much-simplified label- free approach makes the proposed biosensor particularly attractive for routine miRNA analysis. 30,31 To date, all miRNA assays must work with highly purified RNA samples. The freedom from chemical/biological ligation and PCR amplification greatly improves the suitability in direct profiling miRNAs with minimal or no sample pretreatment. Attempts were thus made in utilizing the biosensor for analyzing circulating miRNAs in blood and miRNAs in total RNA extract from culture cells. The same sample (except serum) was simultaneously analyzed by the biosensor and by qPCR. As presented in Table 1, good agreements between the results obtained by the biosensor and qPCR on the same sample confirmed the practical value of the biosensor. In addition, the results obtained were also consistent with published data. 32,33 Because of its high sensitivity, the amount of RNA needed for asuccessful miRNA detection was in the range of 3−10 ng. In addition, a direct analysis of let-7 miRNA in serum was attempted. Unfortunately, persistently lower values with much larger variations (30%−50%) were obtained. The results were even worse when RNA extraction efficiency is taken into consideration. Evidently, the high protein content and other blood constituents in serum severely interfere with the detection of miRNA, probably by fouling the biosensor through nonspecific adsorption. Efforts in developing an antifouling interface while maintaining most of the attractive features of the biosensor are underway. At the present time, this work only serves as a proof-of- concept through rather labor-intensive manual operation. Nonetheless, as with other electronic/electrochemical bio- sensors, both low- and high-density sensor arrays can, in principle, be constructed by adopting the standard micro- fabrication technology and the complementary metal-oxide semiconductor technology that require no additional processes other than a simple switching mechanism. Two prominent examples demonstrating the power and capacity of the electronic/electrochemical detection systems are the electro- chemical microarray developed by combimatrix 34 and the Ion Proton Sequencer recently developed by Life Technologies. 35 For example, up to 12 million individal sensors can be fabricated on a single chip in the Ion Proton Sequencer. Compared to other label-free electrochemical biosensors, 12−15 the much simplified procedure implies that the throughput can also be significantly upscaled by upgrading the manual operation to a microfluidic cartridge. Of course, similar to all other hybridization-based systems, great care must be taken when profiling multiple miRNAs sinutaneously, since there is very little room to fine-tune the hybridization conditions for all miRNAs. |
回復(fù)此樓» 猜你喜歡
303求調(diào)劑
已經(jīng)有8人回復(fù)
-
考研調(diào)劑
已經(jīng)有10人回復(fù)
-
0856材料化工調(diào)劑 總分330
已經(jīng)有8人回復(fù)
-
316求調(diào)劑
已經(jīng)有3人回復(fù)
-
317求調(diào)劑
已經(jīng)有3人回復(fù)
-
材料與化工（0856）304求B區(qū)調(diào)劑
已經(jīng)有4人回復(fù)
-
322求調(diào)劑
已經(jīng)有3人回復(fù)
-
085601 材料工程 313分 求調(diào)劑
已經(jīng)有4人回復(fù)
-
321求調(diào)劑
已經(jīng)有4人回復(fù)
-
329求調(diào)劑
已經(jīng)有3人回復(fù)
yuankanxue
金蟲 (著名寫手)
分子玩家
- 應(yīng)助: 38 (小學(xué)生)
- 金幣: 1006.8
- 散金: 98
- 紅花: 2
- 帖子: 1692
- 在線: 427.1小時
- 蟲號: 595695
- 注冊: 2008-09-08
- 性別: GG
- 專業(yè): 波譜分析與成像分析
2樓2013-05-11 10:18:00
金獅水友
至尊木蟲 (著名寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時
- 蟲號: 1817809
- 注冊: 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
3樓2013-05-11 12:28:03
金獅水友
至尊木蟲 (著名寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時
- 蟲號: 1817809
- 注冊: 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
4樓2013-05-11 13:11:49
yuankanxue
金蟲 (著名寫手)
分子玩家
- 應(yīng)助: 38 (小學(xué)生)
- 金幣: 1006.8
- 散金: 98
- 紅花: 2
- 帖子: 1692
- 在線: 427.1小時
- 蟲號: 595695
- 注冊: 2008-09-08
- 性別: GG
- 專業(yè): 波譜分析與成像分析
5樓2013-05-11 14:54:26
cory0931
至尊木蟲 (文壇精英)
巫山云
- 翻譯EPI: 14
- 應(yīng)助: 28 (小學(xué)生)
- 貴賓: 0.101
- 金幣: 30146.9
- 散金: 13861
- 紅花: 198
- 帖子: 10478
- 在線: 1003.5小時
- 蟲號: 1652657
- 注冊: 2012-02-29
- 性別: GG
- 專業(yè): 臨床獸醫(yī)學(xué)
6樓2013-05-11 21:20:10
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 金獅水友 的主題更新
| 6 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 322求調(diào)劑 +3 | 舊吢 2026-03-24 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 求調(diào)劑323材料與化工 +7 | 1124361 2026-03-24 | 7/350 |
|
|
|
[考研] 0856材料專碩353求調(diào)劑 +9 | NIFFFfff 2026-03-20 | 9/450 |
|
|
|
[考研] 316求調(diào)劑 +5 | 江辭666 2026-03-26 | 5/250 |
|
|
|
[考研] 351求調(diào)劑 +4 | 麥克阿磊 2026-03-24 | 4/200 |
|
|
|
[考研] 調(diào)劑求收留 +7 | 果然有我 2026-03-26 | 7/350 |
|
|
|
[考研] 【雙一流院校新能源、環(huán)境材料，材料加工與模擬招收大量調(diào)劑】 +4 | Higraduate 2026-03-22 | 8/400 |
|
|
|
[考研] 07化學(xué)280分求調(diào)劑 +8 | 722865 2026-03-23 | 8/400 |
|
|
|
[考研] 309求調(diào)劑 +4 | gajsj 2026-03-25 | 5/250 |
|
|
|
[考研] 303求調(diào)劑 +6 | 藍(lán)山月 2026-03-25 | 6/300 |
|
|
|
[考研] 332求調(diào)劑 +6 | 032500 2026-03-25 | 6/300 |
|
|
|
[考研] 0854電子信息求調(diào)劑 +7 | α____ 2026-03-22 | 9/450 |
|
|
|
[考研] 284求調(diào)劑 +15 | Zhao anqi 2026-03-22 | 15/750 |
|
|
|
[考研] 生物學(xué)學(xué)碩求調(diào)劑 +7 | 小羊睡著了? 2026-03-23 | 10/500 |
|
|
|
[考研] 335求調(diào)劑 +4 | yuyu宇 2026-03-23 | 5/250 |
|
|
|
[考研] 336化工調(diào)劑 +4 | 王大坦1 2026-03-23 | 5/250 |
|
|
|
[考研] 298求調(diào)劑 +8 | 上岸6666@ 2026-03-20 | 8/400 |
|
|
|
[考研] 285求調(diào)劑 +6 | ytter 2026-03-22 | 6/300 |
|
|
|
[考研] 初試 317 +7 | 半拉月丙 2026-03-20 | 7/350 |
|
|
|
[考研] 266求調(diào)劑 +3 | 哇呼哼呼哼 2026-03-20 | 3/150 |
|