 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 6 | 1/1 | 返回列表 |
| 查看: 685 | 回復: 5 | |||
金獅水友至尊木蟲 (著名寫手)
|
[求助]
英譯中---生物傳感器論文--求科學的翻譯
|
|
Apart from its high sensitivity, as compared to other electrochemical miRNA biosensors, the much-simplified label- free approach makes the proposed biosensor particularly attractive for routine miRNA analysis. 30,31 To date, all miRNA assays must work with highly purified RNA samples. The freedom from chemical/biological ligation and PCR amplification greatly improves the suitability in direct profiling miRNAs with minimal or no sample pretreatment. Attempts were thus made in utilizing the biosensor for analyzing circulating miRNAs in blood and miRNAs in total RNA extract from culture cells. The same sample (except serum) was simultaneously analyzed by the biosensor and by qPCR. As presented in Table 1, good agreements between the results obtained by the biosensor and qPCR on the same sample confirmed the practical value of the biosensor. In addition, the results obtained were also consistent with published data. 32,33 Because of its high sensitivity, the amount of RNA needed for asuccessful miRNA detection was in the range of 3−10 ng. In addition, a direct analysis of let-7 miRNA in serum was attempted. Unfortunately, persistently lower values with much larger variations (30%−50%) were obtained. The results were even worse when RNA extraction efficiency is taken into consideration. Evidently, the high protein content and other blood constituents in serum severely interfere with the detection of miRNA, probably by fouling the biosensor through nonspecific adsorption. Efforts in developing an antifouling interface while maintaining most of the attractive features of the biosensor are underway. At the present time, this work only serves as a proof-of- concept through rather labor-intensive manual operation. Nonetheless, as with other electronic/electrochemical bio- sensors, both low- and high-density sensor arrays can, in principle, be constructed by adopting the standard micro- fabrication technology and the complementary metal-oxide semiconductor technology that require no additional processes other than a simple switching mechanism. Two prominent examples demonstrating the power and capacity of the electronic/electrochemical detection systems are the electro- chemical microarray developed by combimatrix 34 and the Ion Proton Sequencer recently developed by Life Technologies. 35 For example, up to 12 million individal sensors can be fabricated on a single chip in the Ion Proton Sequencer. Compared to other label-free electrochemical biosensors, 12−15 the much simplified procedure implies that the throughput can also be significantly upscaled by upgrading the manual operation to a microfluidic cartridge. Of course, similar to all other hybridization-based systems, great care must be taken when profiling multiple miRNAs sinutaneously, since there is very little room to fine-tune the hybridization conditions for all miRNAs. |
回復此樓» 猜你喜歡
295材料工程專碩求調劑
已經有3人回復
-
085701環(huán)境工程求調劑
已經有5人回復
-
083000學碩274求調劑
已經有4人回復
-
275求調劑
已經有8人回復
-
295求調劑
已經有4人回復
-
一志愿211院校 344分 東北農業(yè)大學生物學學碩，求調劑
已經有8人回復
-
085602 307分 求調劑
已經有5人回復
-
322求調劑
已經有3人回復
-
高溫高壓反應求助
已經有3人回復
-
一志愿上海理工能源動力（085800）310分求調劑
已經有4人回復
yuankanxue
金蟲 (著名寫手)
分子玩家
- 應助: 38 (小學生)
- 金幣: 1006.8
- 散金: 98
- 紅花: 2
- 帖子: 1692
- 在線: 427.1小時
- 蟲號: 595695
- 注冊: 2008-09-08
- 性別: GG
- 專業(yè): 波譜分析與成像分析
2樓2013-05-11 10:18:00
金獅水友
至尊木蟲 (著名寫手)
- 應助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時
- 蟲號: 1817809
- 注冊: 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
3樓2013-05-11 12:28:03
金獅水友
至尊木蟲 (著名寫手)
- 應助: 9 (幼兒園)
- 金幣: 12278.2
- 散金: 50
- 紅花: 13
- 沙發(fā): 5
- 帖子: 1686
- 在線: 203小時
- 蟲號: 1817809
- 注冊: 2012-05-15
- 性別: GG
- 專業(yè): 功能與智能高分子
4樓2013-05-11 13:11:49
yuankanxue
金蟲 (著名寫手)
分子玩家
- 應助: 38 (小學生)
- 金幣: 1006.8
- 散金: 98
- 紅花: 2
- 帖子: 1692
- 在線: 427.1小時
- 蟲號: 595695
- 注冊: 2008-09-08
- 性別: GG
- 專業(yè): 波譜分析與成像分析
5樓2013-05-11 14:54:26
cory0931
至尊木蟲 (文壇精英)
巫山云
- 翻譯EPI: 14
- 應助: 28 (小學生)
- 貴賓: 0.101
- 金幣: 30146.9
- 散金: 13861
- 紅花: 198
- 帖子: 10478
- 在線: 1003.5小時
- 蟲號: 1652657
- 注冊: 2012-02-29
- 性別: GG
- 專業(yè): 臨床獸醫(yī)學
6樓2013-05-11 21:20:10
相關版塊跳轉
我要訂閱樓主 金獅水友 的主題更新
| 6 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 277跪求調劑 +4 | 1915668 2026-03-27 | 7/350 |
|
|---|---|---|---|---|
|
[考研] 安徽大學專碩生物與醫(yī)藥專業(yè)(086000)324分，英語已過四六級，六級521，求調劑 +4 | 美味可樂雞翅 2026-03-26 | 4/200 |
|
|
|
[考研] 材料求調劑 +8 | @taotao 2026-03-21 | 8/400 |
|
|
|
[考研] 調劑 +3 | 李嘉圖·S·路 2026-03-27 | 3/150 |
|
|
|
[考研] 0703化學一志愿南京師范大學303求調劑 +3 | zzffylgg 2026-03-24 | 3/150 |
|
|
|
[考研] 276求調劑。有半年電池和半年高分子實習經歷 +10 | 材料學257求調劑 2026-03-23 | 11/550 |
|
|
|
[考研]
材料學碩，求調劑
 6+5
|
糖葫蘆888ll 2026-03-22 | 10/500 |
|
|
|
[考研] 316求調劑 +5 | 江辭666 2026-03-26 | 5/250 |
|
|
|
[考研] 343求調劑 +4 | 贈我一本書 2026-03-23 | 4/200 |
|
|
|
[考研] 271求調劑 +6 | 生如夏花… 2026-03-22 | 6/300 |
|
|
|
[考研] 機械學碩310分，數(shù)一英一，一志愿211本科雙非找調劑信息 +3 | @357 2026-03-25 | 3/150 |
|
|
|
[考研] 085601求調劑總分293英一數(shù)二 +4 | 鋼鐵大炮 2026-03-24 | 4/200 |
|
|
|
[考研] 尋找調劑 +5 | 倔強芒? 2026-03-21 | 8/400 |
|
|
|
[考研] 318求調劑 +5 | plum李子 2026-03-21 | 8/400 |
|
|
|
[考研] 一志愿武理085500機械專業(yè)總分300求調劑 +3 | an10101 2026-03-24 | 7/350 |
|
|
|
[考研] 化工專碩求調劑 +3 | question挽風 2026-03-24 | 3/150 |
|
|
|
[考研] 080500求調劑 +3 | zzzzfan 2026-03-24 | 3/150 |
|
|
|
[考研] 335求調劑 +4 | yuyu宇 2026-03-23 | 5/250 |
|
|
|
[考研] 0703化學調劑 +4 | 妮妮ninicgb 2026-03-21 | 4/200 |
|
|
|
[考研] 求助 +5 | 夢里的無言 2026-03-21 | 6/300 |
|