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325865959銅蟲 (初入文壇)
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[求助]
請(qǐng)問有沒有模擬酶分子酸堿耐受性的算法，軟件，或者綜述？
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見過模擬蛋白質(zhì)熱穩(wěn)定性的算法，但是有沒有模擬酶分子酸堿耐受性的算法呢？都是什么原理呀？一般工業(yè)酶改造需要用到提高酸堿耐受范圍，是隨機(jī)突變呢還是有理性設(shè)計(jì)的方法指導(dǎo)？ [ Last edited by 325865959 on 2013-5-24 at 11:22 ] |
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4樓2013-05-27 16:31:18
325865959
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| pKD software: "The pKa values inproteins governthe pH-dependence of protein stability and enzymatic activity. A large number of mutagenesis experiments have been carried out in the last three decades to re-engineer the pH-activity and pH-stability profile of enzymes and proteins. We have developed the pKD webserver (http://polymerase.ucd.ie/pKa_Design), which predicts sets of point mutations that will change the pKa values of a set of target residues in a given direction, thus allowing for targeted re-design of the pH-dependent characteristics of proteins." |
2樓2013-05-27 12:50:53
325865959
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| PROPKA software: "The PROPKA Graphical User Interface (GUI) is a new tool for studying the pH-dependent properties of proteins such as charge and tabilization energy. It facilitates a quantitative analysis of pKa values of ionizable residues together with their structural determinants by providing a direct link between the pKa data, predicted by the PROPKA calculations, and the structure via the Visual Molecular Dynamics (VMD) program." |
3樓2013-05-27 12:55:56
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