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[求助]
關(guān)于做蛋白質(zhì)的問(wèn)題:猝滅常數(shù)和結(jié)合常數(shù)的區(qū)別 已有1人參與
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做蛋白的熒光光譜 我想問(wèn)下,猝滅常數(shù)和結(jié)合常數(shù)對(duì)等么? 猝滅常數(shù)感覺(jué)就是猝滅了多少的意思,不就是藥物結(jié)合蛋白結(jié)合了多少的意思么?是不是兩個(gè)是對(duì)等的呢? |
新蟲(chóng) (初入文壇)
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熒光猝滅也分靜態(tài)和動(dòng)態(tài),靜態(tài)猝滅就是形成復(fù)合物,和你理解意思差不多,動(dòng)態(tài)的稍微少,但是也有。一下是文獻(xiàn)中可找到的參考解釋。It is well known that fluorescence quenching mechanism can be classified as dynamic quenching and static quenching. Dynamic quenching refers to a process that the fluorophore and the quencher come into contact during the transient existence of the excited state, while static quenching refers to fluorophore–quencher complex formation. They can be distinguished by their different dependence features on temperature, and viscosity or, preferable, by lifetime measurements . For static quenching, the quenching constant decreases with increasing temperature, but the reverse effect would be observed for dynamic quenching. |
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