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xiehuaiyan木蟲 (小有名氣)
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[求助]
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The aim of the research was to determine the chlorophyll and carotene pigments simultaneously from different vegetables, using three organic solvents, in order to obtain the best extraction solution. Absorptive properties of the pigments were followed up in quantitative analysis using a spectrophotometric method. The literature presents two methods to determine these pigments - the trichromatic method and monochromatic method, the two methods differing by the used wavelengths. It is known that chlorophyll pigments have broad absorption band from blue to red. Also the coextracted carotenoids have maximum absorption in blue band so that the chosen determination method is based on measuring the absorbance at three wavelengths for each type of matrix and then calculate chlorophyll and carotene using the three "trichromatic equations" of LICHTENTALER and WELLBURN |
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試翻譯如下: 【個(gè)人認(rèn)為原作者語言有些奇怪】 本研究的目的在于使用三種不同的有機(jī)溶劑(【使用三種不同溶劑是】為了獲取最好的萃取液) 在不同植物中同時(shí)測定葉綠素以及胡羅卜素的含量。 【萃取之后】,色素的吸光值緊接著被分光光度法所定量測定。本文獻(xiàn)展現(xiàn)了兩種分析這些色素的方法-三色法(trichromatic)以及單色法(monochromatic),這兩種方法的區(qū)別在于所用波長的不同。已知葉綠素可以吸收由藍(lán)色到綠色的較大范圍的光,而共萃取的胡蘿卜素的最大吸光度是在藍(lán)色(條帶)。 因此所選用的分析方法是根據(jù)測量在三個(gè)不同波長下每個(gè)基質(zhì)的不同的吸光度,運(yùn)用Lichtenthaler 和Wellburn的三色法公式來計(jì)算葉綠素以及胡蘿卜素的含量。 The aim of the research was to determine the chlorophyll and carotene pigments simultaneously from different vegetables, using three organic solvents, in order to obtain the best extraction solution. 本研究的目的在于使用三種不同的有機(jī)溶劑(為了獲取最好的萃取液) 在不同植物中同時(shí)測定葉綠素以及胡羅卜素的含量。 Absorptive properties of the pigments were followed up in quantitative analysis using a spectrophotometric method. 色素的吸光值緊接著被分光光度法所定量測定 The literature presents two methods to determine these pigments - the trichromatic method and monochromatic method, the two methods differing by the used wavelengths. 本文獻(xiàn)呈現(xiàn)了兩種分析這些色素的方法-三色法(trichromatic)以及單色法(monochromatic),這兩種方法的區(qū)別在于所用波長的不同。 It is known that chlorophyll pigments have broad absorption band from blue to red. 已知葉綠素可以吸收由藍(lán)色到綠色的較大范圍的光。 Also the coextracted carotenoids have maximum absorption in blue band so that the chosen determination method is based on measuring the absorbance at three wavelengths for each type of matrix and then calculate chlorophyll and carotene using the three "trichromatic equations" of LICHTENTALER and WELLBURN 同時(shí),共萃取的胡蘿卜素的最大吸光度是在藍(lán)色(條帶)。 因此所選用的分析方法是根據(jù)測量在三個(gè)不同波長下每個(gè)基質(zhì)的不同的吸光度,運(yùn)用Lichtenthaler 和Wellburn的三色法公式來計(jì)算葉綠素以及胡蘿卜素的含量。 |
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