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jghu9200木蟲 (小有名氣)
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[求助]
Reviewer提的兩個問題,求如何回答,如何做?萬分感謝
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Reviewer提的兩個問題,本人對這個問題有點拿不準(zhǔn),求熱心戰(zhàn)友幫忙,如何回答?,如何做?萬分感謝 Reviewer #2: The authors did address most concerns of the reviewer. However, there is one technical issue which has to be clarified. 1. In the MM section, the authors state that "To ensure that the samples were free of genomic DNA, RNA samples were subjected to a reverse transcription in the absence of the reverse transcriptase. Our results didn't find any genomic DNA contamination." The authors are correct to assume that if there is any contamination with genomic DNA, then there should be amplification in the subsequent PCR. HOWEVER, in case the primer is an exon-intron spanning oligonucleotide and the amplified intron is too long, than this genomic contamination band will not be visible in a 1% agarose gel. I strongly suggest performing another control experiment with contaminated RNA to ensure that the assay performed is sufficient to detect genomic DNA contamination. 3. Which strategy was followed to evaluate real-time PCR experiments? |
木蟲 (小有名氣)
木蟲 (小有名氣)
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