版塊導(dǎo)航: 正在加載中...

登錄注冊

應(yīng)《網(wǎng)絡(luò)安全法》要求，自2017年10月1日起，未進(jìn)行實(shí)名認(rèn)證將不得使用互聯(lián)網(wǎng)跟帖服務(wù)。為保障您的帳號能夠正常使用，請盡快對帳號進(jìn)行手機(jī)號驗(yàn)證，感謝您的理解與支持！

24小時熱門版塊排行榜

返回列表

卡西西VS鳴人

金蟲 (小有名氣)

應(yīng)助: 8 (幼兒園)
金幣: 1215.4
散金: 40
紅花: 4
帖子: 172
在線: 22.9小時
蟲號: 2212186
注冊: 2012-12-29
專業(yè): 農(nóng)業(yè)昆蟲學(xué)

[求助] 求助文獻(xiàn) 已有1人參與

Use of acetylcholinesterase activity to detect sublethal toxicity in stream invertebrates exposed to low concentrations of organophosphate insecticides
Kristin E. Day, Ian M. Scott
文獻(xiàn)網(wǎng)站http://www.sciencedirect.com/science/article/pii/0166445X9090021G
接受郵箱：hepin1314@126.com
Abstract
Acetylcholinesterase (AChE) activity was measured in selected species of aquatic invertebrates using two methodologies based on the Ellman technique — a modified kit procedure using the spectrophotometer (BMC procedure) and a microplate assay. Levels of AChE ranged from 12.7 mU/mg protein for Daphnia magna to 96.9 mU/mg protein for Hydropsyche spp. The coefficients of variation (CVs) for the microplate technique were much lower than the CVs for the BMC procedure (e.g., 6.9 to 25.5% vs. 34.6 to 55.4%) and allowed measurements within single whole organisms or individual head capsules.

Exposure of invertebrates to low concentrations of the organophosphate insecticides, azinphosmethyl and fenitrothion, did not result in a significant depression in AChE levels with one exception, i.e. levels of AChE in Hyalella azteca declined to 55.2% of control levels following 24 h exposure to 2.0 μg azinophosmethyl/l. Exposure for longer periods (48 to 96 h) did not result in reduced levels. Exposure of the stonefly, Claassenia sp., to chlorpyrifos significantly lowered AChE levels by 30.7–45.1% at concentrations approaching lethality (⩾40 μg/l). The results indicate that measurement of the activity of AChE in aquatic invertebrates exposed to field concentrations of organophosphate insecticides may be a useful biochemical technique but only for detecting acute toxicity following exposure in the field. In addition, choice of species may be important in the detection of sublethal effects.

[ Last edited by 卡西西VS鳴人 on 2013-10-2 at 08:32 ]

回復(fù)此樓

» 猜你喜歡

一志愿天大材料與化工（085600）總分338 已經(jīng)有4人回復(fù)
085700資源與環(huán)境308求調(diào)劑已經(jīng)有3人回復(fù)
求材料調(diào)劑已經(jīng)有8人回復(fù)
294求調(diào)劑材料與化工專碩已經(jīng)有5人回復(fù)
一志愿華中科技大學(xué)，080502，354分求調(diào)劑已經(jīng)有4人回復(fù)
一志愿吉林大學(xué)材料學(xué)碩321求調(diào)劑已經(jīng)有6人回復(fù)
085410人工智能專碩317求調(diào)劑（0854都可以）已經(jīng)有3人回復(fù)
330求調(diào)劑已經(jīng)有3人回復(fù)
一志愿中海洋材料工程專碩330分求調(diào)劑已經(jīng)有5人回復(fù)
304求調(diào)劑已經(jīng)有5人回復(fù)

1樓 2013-10-02 08:31:32

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

wrs王瑢笙

木蟲 (正式寫手)

應(yīng)助: 0 (幼兒園)
金幣: 3328.4
紅花: 5
沙發(fā): 1
帖子: 310
在線: 42.5小時
蟲號: 2425415
注冊: 2013-04-19
性別: GG
專業(yè): 植物化學(xué)保護(hù)

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★
卡西西VS鳴人: 金幣+10, ★★★★★最佳答案 2014-01-10 10:34:47

你看看是不是這篇文獻(xiàn)！希望對你有幫助！

贊一下(1人)

回復(fù)此樓

» 本帖附件資源列表

歡迎監(jiān)督和反饋：小木蟲僅提供交流平臺，不對該內(nèi)容負(fù)責(zé)。
本內(nèi)容由用戶自主發(fā)布，如果其內(nèi)容涉及到知識產(chǎn)權(quán)問題，其責(zé)任在于用戶本人，如對版權(quán)有異議，請聯(lián)系郵箱：xiaomuchong@tal.com
附件 1 : Useofacetylcholinesteraseactivitytodetectsublethaltoxicity.pdf

2013-12-20 20:39:42, 748.87 K

2樓2013-12-20 20:39:31