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[求助]
關于內生菌抗菌活性的篩選
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前幾天看到一篇外文文獻,其中關于內生菌抗菌活性篩選那段原文: The endophytic fungi were subjected to an antimicrobial assay using a solid medium which permits a rapid and qualitative selection the bioactive microorganisms. Each fungus was cultivated on the surface of PDA into Petri dishes at 30°C for 7 days. Then, disks were cut from the colony with a flamed cork borer (6 mm diameter) and transferred to the surface of the media Müller-Hinton SAB and SAB supplemented with 0.5% olive oil previously spread with bacteria, Candida albicans and Trichophyton rubrum and Malassezia furfur.關于里面提到的那個什么木塞鉆孔器從圓盤上切下菌落再轉移到后面的一些培養(yǎng)基中,這個過程是怎樣的,切下的菌落是正面向上還是背面向上?因為我初學微生物,所以好多不懂,在這求助各位了,謝謝 |
鐵桿木蟲 (著名寫手)
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