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辰心鐵桿木蟲 (小有名氣)
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[求助]
關(guān)于內(nèi)生菌抗菌活性的篩選
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前幾天看到一篇外文文獻(xiàn),其中關(guān)于內(nèi)生菌抗菌活性篩選那段原文: The endophytic fungi were subjected to an antimicrobial assay using a solid medium which permits a rapid and qualitative selection the bioactive microorganisms. Each fungus was cultivated on the surface of PDA into Petri dishes at 30°C for 7 days. Then, disks were cut from the colony with a flamed cork borer (6 mm diameter) and transferred to the surface of the media Müller-Hinton SAB and SAB supplemented with 0.5% olive oil previously spread with bacteria, Candida albicans and Trichophyton rubrum and Malassezia furfur.關(guān)于里面提到的那個什么木塞鉆孔器從圓盤上切下菌落再轉(zhuǎn)移到后面的一些培養(yǎng)基中,這個過程是怎樣的,切下的菌落是正面向上還是背面向上?因?yàn)槲页鯇W(xué)微生物,所以好多不懂,在這求助各位了,謝謝 |
鐵桿木蟲 (著名寫手)
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