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hanye_168鐵蟲 (初入文壇)
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[求助]
糖漿特征物分析（主要針對(duì)蜂蜜摻假，小麥糖漿和木薯糖漿），求教！ 已有1人參與
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| 我是檢測(cè)關(guān)于蜂蜜摻假的，對(duì)于新的糖漿，需要找出其特征物，進(jìn)液質(zhì)，發(fā)現(xiàn)峰分不開，出峰時(shí)間才2分鐘左右，如何可以把峰分開，希望可以給些建議，謝謝！ |
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lifucheng
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2樓2014-01-12 20:10:00
冷觀風(fēng)雨
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3樓2014-03-27 22:24:10
skychen001
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4樓2014-03-30 22:40:28
celan
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從事NMR和有機(jī)化合物結(jié)構(gòu)鑒定
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Honey carbohydrates were analyzed by high performance anion exchange liquid chromatography with constant potential amperometric detection at a nickel-chromium (80:20) alloy electrode. The sugars are oxidized by a surface catalyzed process proposed to involve Ni(II)/Ni(III) oxyhydroxides, which are formed at approximately 0.500 V (Saturated Calomel as reference electrode). The honey content of glucose, fructose, and sucrose is determined in less than 15 minutes per sample. The high sensibility achieved with this alloy electrode allowed for the analysis of disaccharides and oligosaccharides usually present in honey as minor components. The analysis of these carbohydrates may be useful for the determination of the floral origin of honey. |
5樓2014-04-18 08:46:00
celan
專家顧問 (知名作家)
從事NMR和有機(jī)化合物結(jié)構(gòu)鑒定
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DETERMINATION OF SUGAR COMPOSITION IN PORTUGUESE HEATHER HONEYS BY HPLC/RI HPLC analysis of sugars The HPLC method of AOAC (1990) [10] was followed except that acetonitrile-water mobile phase and solvent flow rate used were 80/20 and 1.5 ml/min., respectively, to improve the separation of the individual sugars on the column. Thus, separation of compounds was achieved with an analytical HPLC unit (Gilson), using an refractive index detector and an μ-Bondapak/Carbohydrate (300x4 mm I.D., Waters Associates, nº84038) column. A 20 μl portion of each prepared honey sample was injected and the sugars content was calculated by external standard method using peak areas. A 20 μl aliquot of the mixed sugars standard was injected in duplicate at the beginning, during, and at the end of each day’s run to check retention time and peak area. http://www.apimondia.com/congresses/2001/Papers/080.pdf |
6樓2014-04-18 08:49:59
winder1998
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7樓2017-06-14 15:59:41
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