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[求助]
糖漿特征物分析(主要針對蜂蜜摻假,小麥糖漿和木薯糖漿),求教! 已有1人參與
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| 我是檢測關于蜂蜜摻假的,對于新的糖漿,需要找出其特征物,進液質,發(fā)現峰分不開,出峰時間才2分鐘左右,如何可以把峰分開,希望可以給些建議,謝謝! |

專家顧問 (知名作家)
從事NMR和有機化合物結構鑒定
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DETERMINATION OF SUGAR COMPOSITION IN PORTUGUESE HEATHER HONEYS BY HPLC/RI HPLC analysis of sugars The HPLC method of AOAC (1990) [10] was followed except that acetonitrile-water mobile phase and solvent flow rate used were 80/20 and 1.5 ml/min., respectively, to improve the separation of the individual sugars on the column. Thus, separation of compounds was achieved with an analytical HPLC unit (Gilson), using an refractive index detector and an μ-Bondapak/Carbohydrate (300x4 mm I.D., Waters Associates, nº84038) column. A 20 μl portion of each prepared honey sample was injected and the sugars content was calculated by external standard method using peak areas. A 20 μl aliquot of the mixed sugars standard was injected in duplicate at the beginning, during, and at the end of each day’s run to check retention time and peak area. http://www.apimondia.com/congresses/2001/Papers/080.pdf |
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