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zy4315456新蟲 (小有名氣)
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[求助]
論文修回,求助專業(yè)人士統(tǒng)計問題 已有1人參與
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我的一篇文章投在Veterinary Journal上,大修的時候有一個審稿人認為我們的統(tǒng)計方法有問題,但我們認為是對的,修回的時候沒有改,舉了許多相關(guān)證據(jù)說服審稿人,F(xiàn)在再次修回,其他的基本沒問題了,但是審稿人還是堅持我們的統(tǒng)計不合理,但是留給雜志社做決定。然后雜志社又找了一個專門搞統(tǒng)計的專家,結(jié)果還是存在爭議,要求我們找精通統(tǒng)計的人給我們意見,但身邊的人對統(tǒng)計都不是太精通。素聞小木蟲高人多,還望專業(yè)人士能夠幫忙指點,小女子不甚感激! 我們的實驗設(shè)計非常簡單,就是隨機區(qū)組設(shè)計,10頭動物分成兩組,一組對照組,一組處理組;主要結(jié)果就是揮發(fā)性脂肪酸,某幾個蛋白的Western結(jié)果,切片的統(tǒng)計結(jié)果(每頭動物做了8張片子,每張片子上選5個點,相當于一頭動物有40個數(shù)據(jù))。請教大家這種情況應該怎么統(tǒng)計,尤其是切片的結(jié)果,希望能有詳細解答。我們最初是用的 independent t-test (unpaired t-test) 下面是審稿人和統(tǒng)計專家的意見: 第一修回意見: Comment: (20) Line 169: is a t-test the most appropriate? Any repeated measures. Better use ANOVA and five the model. Also were any data transformations needed? 第二次修回意見:I am insure about the appropriateness of the statistical analysis, but I leave that for the journal to decide. 雜志找的 統(tǒng)計專家意見:It was clearly stated that there were five goats in either the hay or HG diet. However, it was unclear how many specimens per animal were available for the microscopic study. Two sample t-test might be a reasonable statistical method to assess the difference between hay and HG groups (n=5 for both groups) when each goat had only one measurement. However, this is not an appropriate method when you have repeated measures from the same animal (omasal epithelial thickness measurements in Table 2, n=200) [ Last edited by cxksama on 2014-2-23 at 11:51 ] |
新蟲 (小有名氣)
新蟲 (小有名氣)
| 你這種情況屬于典型的重復測量(repeated measures):因為每頭動物有40個數(shù)據(jù)點,這40個數(shù)據(jù)點之間的變異情況屬于被試內(nèi)變異(within-subject variance),區(qū)別于動物與動物之間的變異(between-subject variance),需要在統(tǒng)計模型中體現(xiàn)出來,而合適的統(tǒng)計模型就是repeated-measures ANOVA。我給你上傳了在SPSS中如何做這種統(tǒng)計的文件,你看看吧。 |
新蟲 (小有名氣)
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