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[求助]
有沒有人知道detergent-resistant membranes 已有2人參與
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| 細胞膜上有抗去垢劑區(qū)域嗎？detergent-resistant membranes，有沒有大神懂得給解釋一下，抗去垢劑膜和病毒內(nèi)吞有何聯(lián)系？因為一篇文章中說病毒的一些非結構蛋白會和抗去垢劑膜相聯(lián)系，感謝。。。 |
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2樓2014-06-12 19:16:49
【答案】應助回帖
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History • 80’s Order (package) and disorder phases in diff. parts of the PM. • 90’s Analysis of membrane fractions by the use of mild detergents. The concept of Detergent Resistant Membranes (DRM) born. In this technique non-solubilized fraction of the PM extracts by mild detergent (triton X-100 at 4ºC –artifact conditions-) + sucrose gradient • 2000’s New microscopy techniques: FRET, STED, FRAP… To understand the dynamic of the assembly of sterol, sphingolipds and proteins in living cells (real conditions). Membrane domain definition: Lateral compartment (PM plane) with composition, structure and biological function diff. from the surrounding membrane. Regardless size, stability and mechanism of formation. Why DRMs/DIMs (detergent insoluble microdomains) are not lipid rafts??? • 4ºC is not a real condition for the behavior of the PM. • Quantitative bias due the ratio concentration of detergent:prot. /detergent:lipids Definition by consensus 2006: “Membrane rafts are small (10-200nm), heterogeneous highly dynamic, sterol and sphingolipid-enriched domains that compartmentalized cellular process” What is new here: No: detergent resistant, lipid term, or PM (E.g. membrane of mitochondria), small (need of superesolution techniques). |
3樓2014-06-12 19:20:22
4樓2019-06-04 12:17:08
5樓2019-06-04 12:21:45
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