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zbr405新蟲 (初入文壇)
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[求助]
審稿意見求助 已有2人參與
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各位蟲友,我投的是Sensors and Actuators B, 17天就返回了審稿意見，大家?guī)蛶兔Γ瑤臀依斫庖幌聦徃迦说囊馑际鞘裁�，審稿意見如下�?br />
Reviewers' comments: Reviewer #1: In this paper, the author prepared electrochemiluminescence aptasensing sensor based on ferrocene-graphene nanosheets for the detection of thrombin. However, the following issues need to be addressed before accept for publication: (1) The English of the manuscript needs improvement. (2) In the paper, the author said the ECL intensity of the Ru(bpy)32+ was quenched by Fc, but through the paper, the author did not prove the ECL intensity was actually quenched by Fc or graphene, as graphene also is a quencher. (3) In the experimental section, the author needs to briefly state how Fc-GNs were synthesized. (4) In fig. 6, what the author mean ECL intensity vs. time curves? Why not use the traditional ECL intensity vs. potential curves? (5) Statistical dada are missing in the figs with error bars, how many tests are repeated? Reviewer #2: This manuscript is a report containing innovation with new data for detection of thrombin. It is worthy to be published after some minor revisions. Some suggestions are listed as followings: 1. The voltage supplied to the photomuliplier for luminescence intensity determination needs to be confirmed. 2. A brief description about the mechanism of ECL through TPrA is better to be included in the manuscript. 3. A possible explanation for the optimal pH to give best activity is suggested. 4. The Nyquist plot was used to demonstrate a increase in charge transfer resistance after the formation of the insulating Ru-TBA layer. For a insulating layer, the electron resistance should also increase and showed an increase in the intercept with the Z' axis at high frequency. The manuscript is better to show this behavior. 5. Figure 5 shows that the ECL intensity change, <DELTA>IECL, was about 100 for Fc-GNs/Ru-TBA modified GCE. But, the ECL intensity change was about 300 without thrombin according calibration curve in Figure 6. Is there any explanation for this difference? |
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F_Shaw
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2樓2014-06-18 11:04:52
carefu
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【答案】應(yīng)助回帖
★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
zbr405: 金幣+3, 謝謝 2014-06-18 14:52:48
感謝參與，應(yīng)助指數(shù) +1
zbr405: 金幣+3, 謝謝 2014-06-18 14:52:48
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我嘗試?yán)斫庖幌掳桑?br />
審稿人1： 1、英語太爛了，得改。 2、你直接提出來一種解釋，卻沒有實驗來證實。（這個，如果實驗實在做不出來，可以說由于實驗條件限制什么的，然后找?guī)灼嚓P(guān)的文獻(xiàn)引證一下就行） 3、簡要說明制備方法。 4.為什么要用密度時間曲線？ 5、這個應(yīng)該是說你的統(tǒng)計曲線里，只有誤差棒，但是把原來的實驗數(shù)據(jù)給隱藏了。還有，要額外點明統(tǒng)計了多少次實驗的數(shù)據(jù)。 審稿2 ： 1、好像是測定光強吧，應(yīng)該是補數(shù)據(jù)的意思 2、簡述機制 3、解釋活性最佳ph值。 4、審稿人提出來一種什么現(xiàn)象或解釋之類的，我是外行，不懂，樓主自己理解吧。 5、Fig5和6不符，要求給個解釋。 感覺都不難回答，也沒有特別犀利的問題。希望很大的 |
3樓2014-06-18 11:21:09
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