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liduoli新蟲(chóng) (初入文壇)
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[求助]
感受態(tài)Stable4求助 已有1人參與
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| 大家有沒(méi)有Stable4感受態(tài)��？急需�。】梢越粨Q的！ |
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飛約瘋?cè)嗽?/a>
專家顧問(wèn) (著名寫(xiě)手)
科學(xué)怪人
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專家經(jīng)驗(yàn): +140 - MolEPI: 2
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- 帖子: 1996
- 在線: 266.1小時(shí)
- 蟲(chóng)號(hào): 3188421
- 注冊(cè): 2014-05-07
- 專業(yè): 生物化學(xué)
- 管轄: 分子生物
【答案】應(yīng)助回帖
★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
liduoli(西門(mén)吹雪170代發(fā)): 金幣+3, 鼓勵(lì)回帖交流 2014-06-23 18:30:46
感謝參與，應(yīng)助指數(shù) +1
liduoli(西門(mén)吹雪170代發(fā)): 金幣+3, 鼓勵(lì)回帖交流 2014-06-23 18:30:46
下面是國(guó)內(nèi)能買(mǎi)到的地方，只有(Invitrogen™ 有：ElectroMAX™ Stbl4™ Competent Cells（貨號(hào)是11635-018） 描述 ElectroMAX™ Stbl4™ Competent Cells are specifically designed for cloning unstable inserts. As electrocompetent cells, they have one of the highest transformation efficiencies available (>5×109 cfu/µg), making them ideal for generating cDNA and genomic libraries and for cloning unstable inserts. These electrocompetent E. coli: • Are ideal for cloning unstable DNA—stabilizes direct repeat and retroviral sequences • Permit efficient cloning of methylated genomic DNA • Support blue/white screening • Are designed to deliver high-yield plasmid preparations for downstream applications • Provide transformation efficiencies of >5×109 cfu/µg Propagating unstable and large DNA with high transformation-efficiency cells Many competent cell strains have the recA1 genotype, which reduces recombination. However there are some instances when the DNA that you are trying to clone is still unstable in such cells, perhaps due to the presence of inverted or direct repeats, or GC-rich tracts. While such sequences are relatively common in eukaryotic genomes, they are rare in E. coli. Consequently, rearrangements may occur when these sequences are introduced into standard E. coli strains. Using ElectroMAX™ Stbl4™ Cells ElectroMAX™ Stbl4™ electrocompetent E. coli cells are a derivative of Stbl2™ cells and are ideal for the cloning of unstable inserts such as retroviral sequences, direct repeats, and tandem array genes. Furthermore, ElectroMAX™ Stbl4™ cells are useful for the generation of cDNA libraries using plasmid-derived vectors and are able to take up and maintain large plasmids (e.g., 50 kb cosmids and 100–200 kb P1 clones). The Stbl4™ cells also contain an F' episome, allowing them to serve as a host for single-stranded DNA such as M13mp cloning vectors. The lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can therefore be used for blue/white screening of colonies on agar plates containing Xgal or Bluo-gal and IPTG. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow cloning of genomic sequences which are methylated. Finally, the endA1 mutation greatly increases plasmid yield and quality. Genotype: mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+lacIqZΔM15 Tn10 (TetR) 下面是聯(lián)系方式 Life Technologies 上海辦事處 英濰捷基（上海）貿(mào)易有限公司 地址：上海市長(zhǎng)寧區(qū)仙霞路99號(hào)尚嘉中心21-22樓 郵編：200051 電話：021-61452000 傳真：021-64482250 |
2樓2014-06-23 16:43:59
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