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利用分子伴侶GroEL的天然雙環(huán)結(jié)構(gòu)自組裝成納米管，可用于生物醫(yī)藥領(lǐng)域。。 文獻(xiàn)制備方法簡單，表征手段前沿，可以作為蛋白納米材料方向研究生的參考資料 1. Self-assembly of the chaperonin GroEL nanocage induced at submicellar detergent Scientific Reports http://www.nature.com/srep/2014/ ... full/srep05614.html Volume: 4,Article number:5614 DOI: doi:10.1038/srep05614 Published08 July 2014 Protein nanoassemblies possess unique advantage in biomedical applications such as drug delivery, biocatalysis and vaccine development. Despite recent accomplishment in atomic structure data, the underlying molecular mechanism of protein self-assembly remains elusive, where considerable heterogeneity is often involved. Here we use E. coli chaperonin GroEL, a tetradecameric protein with a molecular weight of 805 kDa, to probe its transformation from cage-like oligomers to protein nanofibers. We show that sodium dodecyl sulfate (SDS), a widely-used protein denaturant, at submicellar concentration binds to and causes partial distortion of GroEL apical domain. Subsequently, the GroEL apical domain with altered secondary structural content converts the GroEL oligomers into modular structural units which are observed to self-assemble into cylindrical nanofibers under an agitated incubation in a physiological buffer. Interestingly, through targeted mutagenesis where two cysteine residues are introduced at the entry site of GroEL cage, we found that the formation of GroEL nanoassembly could be modulated depending on the redox condition of incubation. Without the need of chemical engineering, tunable GroEL nanofibers built by controlled-assembly are among the largest nanoscale bioassembly with broad applications. 2. A Tubular Biocontainer: Metal Ion-Induced 1D Assembly of a Molecularly Engineered Chaperonin J. Am. Chem. Soc., 2009, 131 (22), pp 7556–7557 DOI: 10.1021/ja902696q Publication Date (Web): May 12, 2009 http://pubs.acs.org/doi/abs/10.1021/ja902696q GroELSP/MC, prepared by genetic and chemical modifications of group I chaperonin protein GroEL, site-specifically possesses 28 photochromic (spiropyran [SP] and merocyanine [MC]) units in the entrance parts of its cavity. Addition of divalent metal ions such as Mg2+ to a tris-HCl buffer solution of GroELSP/MC results in one-dimensional (1D) assembly of GroELSP/MC, affording cylindrical hollow fibers with a very large aspect ratio; the longest fiber was 2.5 μm long, corresponding to a 170-mer of GroELSP/MC (MW ≈ 1.4 × 108). When such long fibers are mixed with EDTA, they are cut into short-chain oligomers and eventually into monomeric GroELSP/MC. Similar to GroEL, GroELSP/MC possesses a large binding affinity toward denatured proteins. When GroELSP/MC undergoes 1D assembly after incubation with a denatured protein, guest-containing cylindrical fibers result. |
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歡迎監(jiān)督和反饋：小木蟲僅提供交流平臺，不對該內(nèi)容負(fù)責(zé)。
本內(nèi)容由用戶自主發(fā)布，如果其內(nèi)容涉及到知識產(chǎn)權(quán)問題，其責(zé)任在于用戶本人，如對版權(quán)有異議，請聯(lián)系郵箱：xiaomuchong@tal.com - 附件 1 : 2014_srep05614.pdf
- 附件 2 : 2009_JACS.pdf
2014-07-30 23:44:53, 6.2 M
2014-07-30 23:45:13, 1.41 M
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