版塊導(dǎo)航: 正在加載中...

登錄注冊

應(yīng)《網(wǎng)絡(luò)安全法》要求，自2017年10月1日起，未進(jìn)行實(shí)名認(rèn)證將不得使用互聯(lián)網(wǎng)跟帖服務(wù)。為保障您的帳號能夠正常使用，請盡快對帳號進(jìn)行手機(jī)號驗(yàn)證，感謝您的理解與支持！

24小時(shí)熱門版塊排行榜

返回列表

本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看

AnnF

版主 (文壇精英)

翻譯EPI: 6
應(yīng)助: 197 (高中生)
貴賓: 0.881
金幣: 443225
散金: 5581
紅花: 2313
沙發(fā): 3
帖子: 27689
在線: 3136.6小時(shí)
蟲號: 1223787
注冊: 2011-03-07
專業(yè): 藥物化學(xué)
管轄: 醫(yī)藥類考試

[求助] 【藥理】求助大神翻譯一段話，謝謝！

DH-PS induced the secretions of multiple cytokines and
chemokines in vivo
In order to understand the immunological activities of DH-PS,
we investigated the profiles of cytokines and chemokines induced
by DH-PS in mice. BALB/c mice (n= 3 for DH-PS and phosphate
buffered saline (PBS) group) were injected intraperitoneally (IP)
with DH-PS (300 mg/mouse) in PBS or PBS only and the sera
were collected at 0 (before injection), 2 and 18 hrs after the
injection of DH-PS for the detection of cytokines and chemokines,
which were quantified by Beadlyte Mouse 21-plex Cytokine
Detection system and ELISA kits (for IL-1ra). Compared with PBS
group shown in Figure 1, the increase of TNF-a, IL-12 p40, IL-6,
IL-10 and RANTES were observed at 2 hrs, along with robust
inductions of KC, MCP-1 and MIP-1b. An apparent increase in
IL-1b at 2 hrs was noted but it did not reach statistical significance.
These results suggested that DH-PS induced Th1 (IL-12 p40), Th2
(IL-6, IL-10), inflammatory cytokines (TNF-a) and chemokines
(KC, RANTES, MCP-1, MIP-1b), which might further modulate
the downstream activations of immune cells. Furthermore, an antiinflammatory
molecule, IL-1ra, was increased from 465 to
4199 pg/ml (,9 folds of PBS control group) at 2 hrs. This
indicated that the amounts of IL-1ra induced were 10 folds more
than IL-1b (increased from 144 to 397 pg/ml, ,1.6 folds of PBS
control group), suggesting that DH-PS-induced IL-1ra might override
the activity induced by IL-1b.

1.jpg

回復(fù)此樓

» 猜你喜歡

一志愿北京化工大學(xué)070300 學(xué)碩336求調(diào)劑已經(jīng)有8人回復(fù)
一志愿東華大學(xué)化學(xué)070300，求調(diào)劑已經(jīng)有6人回復(fù)
318求調(diào)劑已經(jīng)有7人回復(fù)
求調(diào)劑一志愿海大，0703化學(xué)學(xué)碩304分，有大創(chuàng)項(xiàng)目，四級已過已經(jīng)有6人回復(fù)
一志愿武理材料工程348求調(diào)劑已經(jīng)有5人回復(fù)
求調(diào)劑已經(jīng)有7人回復(fù)
275求調(diào)劑已經(jīng)有6人回復(fù)
招08考數(shù)學(xué) 已經(jīng)有7人回復(fù)
303求調(diào)劑已經(jīng)有6人回復(fù)
306求調(diào)劑已經(jīng)有4人回復(fù)

1樓 2014-09-19 20:24:53

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

hym0411

木蟲 (正式寫手)

翻譯EPI: 1
應(yīng)助: 16 (小學(xué)生)
金幣: 3135.6
散金: 50
紅花: 5
帖子: 760
在線: 221.6小時(shí)
蟲號: 1403571
注冊: 2011-09-15
性別: MM
專業(yè): 免疫學(xué)

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
RXMCDM: 金幣+1, 多謝應(yīng)助！ 2014-09-19 23:20:43
AnnF: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案, 謝謝，遲來的獎(jiǎng)勵(lì) 2014-09-21 11:51:09

自己翻譯的，紕漏眾多，敬請指正，相互交流，共同進(jìn)步，望采納。
關(guān)于DH-PS在體內(nèi)誘導(dǎo)多種細(xì)胞因子和趨化因子的分泌的研究
為了闡明DH-PS的免疫學(xué)活性，我們研究了DH-PS在小鼠體內(nèi)誘導(dǎo)的細(xì)胞因子和趨化因子的特性。BALB/c小鼠（DH-PS磷酸鹽緩沖液組，3只）用DH-PS磷酸鹽緩沖液（300mg/只）腹腔注射，磷酸鹽緩沖液單獨(dú)注射做對照，并在注射前，注射后2小時(shí)、18小時(shí)分別取血清，用于檢測細(xì)胞因子和趨化因子。定量方法為Beadlyte Mouse 21-plex Cytokine Detection system（儀器）和酶聯(lián)免疫試劑盒。試劑組和對照組的比較見圖一，在2小時(shí)時(shí)，TNF-α，IL-12p40,IL-6,IL-10和RANTES都有增加，而且能夠強(qiáng)烈的誘導(dǎo)產(chǎn)生KC，MCP-1和MIP-1等趨化因子。2小時(shí)時(shí)IL-1b的增加很明顯，但并沒有達(dá)到統(tǒng)計(jì)學(xué)意義上的顯著性差異。這些表明DH-PS能夠誘導(dǎo)產(chǎn)生Th1（IL-12p40）,Th2(IL-6,IL-10)，炎癥性細(xì)胞因子（TNF-α）和趨化因子（KC，RANTES，MCP-1，MIP-1b）等，而這些因子可能會進(jìn)一步調(diào)節(jié)其下游免疫細(xì)胞的活性。而且，抗炎分子IL-1ra在2小時(shí)的血清含量從465pg/ml增加到4199pg/ml（是對照組的9倍）。這表明誘導(dǎo)產(chǎn)生的IL-1ra的量可能會是IL-1b的10倍之多（IL-1b從144pg/ml增加到397pg/ml，是對照組的1.6倍），預(yù)示著DH-ps誘導(dǎo)產(chǎn)生的IL-1ra可能會掩蓋IL-1b誘導(dǎo)產(chǎn)生的活性。

贊一下

回復(fù)此樓

生而無涯

2樓2014-09-19 23:00:16

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

相關(guān)版塊跳轉(zhuǎn) 我要訂閱樓主 AnnF 的主題更新