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本帖產(chǎn)生 1 個翻譯EPI ，點擊這里進行查看

AnnF

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翻譯EPI: 6
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貴賓: 0.881
金幣: 443225
散金: 5581
紅花: 2313
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帖子: 27689
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注冊: 2011-03-07
專業(yè): 藥物化學
管轄: 醫(yī)藥類考試

[求助] 【藥理】求助大神翻譯一段話，謝謝！

DH-PS induced the secretions of multiple cytokines and
chemokines in vivo
In order to understand the immunological activities of DH-PS,
we investigated the profiles of cytokines and chemokines induced
by DH-PS in mice. BALB/c mice (n= 3 for DH-PS and phosphate
buffered saline (PBS) group) were injected intraperitoneally (IP)
with DH-PS (300 mg/mouse) in PBS or PBS only and the sera
were collected at 0 (before injection), 2 and 18 hrs after the
injection of DH-PS for the detection of cytokines and chemokines,
which were quantified by Beadlyte Mouse 21-plex Cytokine
Detection system and ELISA kits (for IL-1ra). Compared with PBS
group shown in Figure 1, the increase of TNF-a, IL-12 p40, IL-6,
IL-10 and RANTES were observed at 2 hrs, along with robust
inductions of KC, MCP-1 and MIP-1b. An apparent increase in
IL-1b at 2 hrs was noted but it did not reach statistical significance.
These results suggested that DH-PS induced Th1 (IL-12 p40), Th2
(IL-6, IL-10), inflammatory cytokines (TNF-a) and chemokines
(KC, RANTES, MCP-1, MIP-1b), which might further modulate
the downstream activations of immune cells. Furthermore, an antiinflammatory
molecule, IL-1ra, was increased from 465 to
4199 pg/ml (,9 folds of PBS control group) at 2 hrs. This
indicated that the amounts of IL-1ra induced were 10 folds more
than IL-1b (increased from 144 to 397 pg/ml, ,1.6 folds of PBS
control group), suggesting that DH-PS-induced IL-1ra might override
the activity induced by IL-1b.

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1樓 2014-09-19 20:24:53

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hym0411

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翻譯EPI: 1
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專業(yè): 免疫學

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
RXMCDM: 金幣+1, 多謝應(yīng)助！ 2014-09-19 23:20:43
AnnF: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案, 謝謝，遲來的獎勵 2014-09-21 11:51:09

自己翻譯的，紕漏眾多，敬請指正，相互交流，共同進步，望采納。
關(guān)于DH-PS在體內(nèi)誘導(dǎo)多種細胞因子和趨化因子的分泌的研究
為了闡明DH-PS的免疫學活性，我們研究了DH-PS在小鼠體內(nèi)誘導(dǎo)的細胞因子和趨化因子的特性。BALB/c小鼠（DH-PS磷酸鹽緩沖液組，3只）用DH-PS磷酸鹽緩沖液（300mg/只）腹腔注射，磷酸鹽緩沖液單獨注射做對照，并在注射前，注射后2小時、18小時分別取血清，用于檢測細胞因子和趨化因子。定量方法為Beadlyte Mouse 21-plex Cytokine Detection system（儀器）和酶聯(lián)免疫試劑盒。試劑組和對照組的比較見圖一，在2小時時，TNF-α，IL-12p40,IL-6,IL-10和RANTES都有增加，而且能夠強烈的誘導(dǎo)產(chǎn)生KC，MCP-1和MIP-1等趨化因子。2小時時IL-1b的增加很明顯，但并沒有達到統(tǒng)計學意義上的顯著性差異。這些表明DH-PS能夠誘導(dǎo)產(chǎn)生Th1（IL-12p40）,Th2(IL-6,IL-10)，炎癥性細胞因子（TNF-α）和趨化因子（KC，RANTES，MCP-1，MIP-1b）等，而這些因子可能會進一步調(diào)節(jié)其下游免疫細胞的活性。而且，抗炎分子IL-1ra在2小時的血清含量從465pg/ml增加到4199pg/ml（是對照組的9倍）。這表明誘導(dǎo)產(chǎn)生的IL-1ra的量可能會是IL-1b的10倍之多（IL-1b從144pg/ml增加到397pg/ml，是對照組的1.6倍），預(yù)示著DH-ps誘導(dǎo)產(chǎn)生的IL-1ra可能會掩蓋IL-1b誘導(dǎo)產(chǎn)生的活性。

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