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Romaner007金蟲 (小有名氣)
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[求助]
求助關(guān)于shRNA的問題,做基因表達的圍觀一下吧,給點建議
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atggctcgc atcaatcgtc ctgctccagt ggaggtttgttacaagaaca tgcgcttcct gatcacccac aatcccacca atgcaacaat gaataccttc atagaggatc tgaagaaata tggcgccacc acggtggtga gggtgtgcga gattacgtac gacaagacgc ccctagagaa ggacggcatc actgtaatgg actggccgtt cgatgatggg gctccacctc ccaataaaat tgtcgatgat tggttgaatt tactgaagac gaaattctgt gaagatcctg gctgctgcgt agctgttcat tgtgtggccg gtttgggaag ggcgccggtc ctggtcgctc ttgcacttat cgagagtggg atgaaatatg aagatgccat tcagttcatc agacagaagc gccggggggc cattaacagc aagcagctga cgtacctgga gaaataccgg ccgaagcaga ggctacgatt caaggatccg cacaatcaca agaacaaatg ctgcatcatg taa CDS of gene X002 includes start code ATG and stop code TAA above. The sequence was analised by Block-It online and best target sequences have been suggested as: GCGAGATTACGTACGACAA GCTGACGTACCTGGAGAAA Please take one and design shRNA depending on vector pSilencer 3.1 for knocking-down the gene expression in stable pattern after transfection. The loop sequence between sense and anti-sense is: TTCAAGAGA. The required vector is linearized with sticky ends and is ready for use. If you have any more questions please ask me without hesitation. BamHI GGATCC, CCTAGG HinDIII AAGCTT TTCGAA |

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