 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 8 | 1/1 | 返回列表 |
| 查看: 2756 | 回復(fù): 7 | ||
z631535213木蟲 (正式寫手)
|
[求助]
關(guān)于細(xì)胞裂解的一點(diǎn)問題 已有3人參與
|
|
準(zhǔn)備自己配點(diǎn)裂解液用用，看了些東西，發(fā)現(xiàn)一些裂解液中提到有效裂解成分，我目前只看到三個(gè)，Triton X-100，NP40，SDS。 想問下對(duì)這方面有了解的蟲友 1.曲通，NP40，SDS一般多少濃度可有效裂解細(xì)胞。主要是用來裂解細(xì)胞提取DNA來進(jìn)行后面的實(shí)驗(yàn) 2.曲通，NP40，SDS對(duì)細(xì)胞的裂解作用能具體講一講么。 恩，大概就這些。拜謝  |
回復(fù)此樓» 猜你喜歡
不合理蛙科研實(shí)驗(yàn)中的趣事：實(shí)驗(yàn)器材的 “烏龍”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)中的趣事：和實(shí)驗(yàn)材料的 “斗智斗勇”
已經(jīng)有0人回復(fù)
-
化學(xué)工程及工業(yè)化學(xué)論文潤(rùn)色/翻譯怎么收費(fèi)?
已經(jīng)有57人回復(fù)
-
蛋白質(zhì)檢測(cè)：精準(zhǔn)分析，解鎖生物分子的密碼
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之小鼠實(shí)驗(yàn)：嚴(yán)謹(jǐn)設(shè)計(jì)，解析生命機(jī)制的重要載體
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之重金屬檢測(cè)：精準(zhǔn)篩查，守護(hù)健康與環(huán)境的防線
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之“蛙測(cè)重金屬我背鍋三千”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之“鼠逃三次我發(fā)三篇SCI”
已經(jīng)有0人回復(fù)
-
納米粒度分析
已經(jīng)有3人回復(fù)
-
林科院林化所招收材料與化工專業(yè)碩士，坐標(biāo)南京
已經(jīng)有0人回復(fù)
» 本主題相關(guān)價(jià)值貼推薦，對(duì)您同樣有幫助:
- 有木有誰建立細(xì)胞株的呀，求經(jīng)驗(yàn)
已經(jīng)有16人回復(fù)
- 動(dòng)物組織研磨和裂解問題
已經(jīng)有13人回復(fù)
- 關(guān)于293T細(xì)胞的問題，急求助
已經(jīng)有43人回復(fù)
- 170kDa膜蛋白MRP1跑WB遇到問題
已經(jīng)有39人回復(fù)
- ALP活性檢測(cè)中細(xì)胞裂解問題
已經(jīng)有10人回復(fù)
- 關(guān)于懸浮細(xì)胞MTT的一點(diǎn)疑問，求解答！
已經(jīng)有11人回復(fù)
- 求助：在不破壞組蛋白結(jié)構(gòu)的前提下，應(yīng)該采用什么樣的細(xì)胞裂解方法？
已經(jīng)有12人回復(fù)
- 求助怎樣使細(xì)菌細(xì)胞裂解但胞內(nèi)的蛋白（例如酶）不失活？
已經(jīng)有9人回復(fù)
- 細(xì)胞裂解提取細(xì)胞內(nèi)的總蛋白，需要使用什么試劑盒，求推薦
已經(jīng)有14人回復(fù)
- 細(xì)胞裂解緩沖液的配制？求助各位大蝦
已經(jīng)有3人回復(fù)
- 關(guān)于THP-1細(xì)胞的培養(yǎng)，分化問題
已經(jīng)有12人回復(fù)
- 關(guān)于源內(nèi)裂解的問題
已經(jīng)有3人回復(fù)
- 關(guān)于質(zhì)譜裂解規(guī)律的問題
已經(jīng)有6人回復(fù)
- 求助裂解細(xì)胞又不降低酶活
已經(jīng)有5人回復(fù)
- 【求助/交流】ALP的定量檢測(cè)一些問題
已經(jīng)有6人回復(fù)
- 【求助/交流】請(qǐng)各位幫幫忙~~細(xì)胞裂解液如何保存，能保存多長(zhǎng)時(shí)間？
已經(jīng)有8人回復(fù)
- 【求助/交流】為什么條帶很細(xì)啊？郁悶?zāi)兀?/a>
已經(jīng)有9人回復(fù)
- 【求助/交流】細(xì)胞裂解的問題
已經(jīng)有19人回復(fù)
- 【求助】關(guān)于裂解氣質(zhì)聯(lián)用分析的問題
已經(jīng)有4人回復(fù)
z631535213
木蟲 (正式寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 2458.4
- 紅花: 3
- 帖子: 479
- 在線: 113.5小時(shí)
- 蟲號(hào): 2756319
- 注冊(cè): 2013-10-27
- 性別: GG
- 專業(yè): 基因組學(xué)
2樓2014-10-17 08:28:43
jurkat.1640
至尊木蟲 (文壇精英)
- MolEPI: 1
- 應(yīng)助: 908 (博后)
- 金幣: 17168.2
- 散金: 1654
- 紅花: 121
- 沙發(fā): 41
- 帖子: 14522
- 在線: 2184.3小時(shí)
- 蟲號(hào): 514340
- 注冊(cè): 2008-02-28
- 性別: GG
- 專業(yè): 病毒學(xué)
3樓2014-10-17 08:58:00
z631535213
木蟲 (正式寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 2458.4
- 紅花: 3
- 帖子: 479
- 在線: 113.5小時(shí)
- 蟲號(hào): 2756319
- 注冊(cè): 2013-10-27
- 性別: GG
- 專業(yè): 基因組學(xué)
4樓2014-10-17 10:31:23
zyd334
新蟲 (小有名氣)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 557.5
- 帖子: 71
- 在線: 60.8小時(shí)
- 蟲號(hào): 3492225
- 注冊(cè): 2014-10-22
- 性別: MM
- 專業(yè): 昆蟲學(xué)
5樓2014-10-22 21:34:59
6樓2014-10-23 10:03:54
【答案】應(yīng)助回帖
|
Detergents are amphipathic molecules, meaning they contain both a nonpolar "tail" having aliphatic or aromatic character and a polar "head". Ionic character of the polar head group forms the basis for broad classification of detergents; they may be ionic (charged, either anionic or cationic), nonionic (uncharged) or zwitterionic (having both positively and negatively charged groups but with a net charge of zero). Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein:protein interaction. Non-denaturing detergents can be divided into nonionic detergents such as Triton* X-100, bile salts such as cholate and zwitterionic detergents such as CHAPS. A major factor determining the behavior and interaction of molecules in biological samples is their hydrophilicity or hydrophobicity. Most proteins and other molecules with charged or polar functional groups are soluble (or miscible) in water because they participate in the highly ordered, hydrogen-bonded intermolecular structure of water. Some other proteins (or at least parts of proteins), as well as fats and lipids, lack polar or charged functional groups; consequently, they are excluded from the ordered interaction of water with other polar molecules and tend to associate together in structures having minimal surface area contact with the polar environment. This association of nonpolar molecules in aqueous solutions is commonly called hydrophobic attraction. Generally, moderate concentrations of mild (i.e., nonionic) detergents compromise the integrity of cell membranes, thereby facilitating lysis of cells and extraction of soluble protein, often in native form. Using certain buffer conditions, various detergents effectively penetrate between the membrane bilayers at concentrations sufficient to form mixed micelles with isolated phospholipids and membrane proteins. Detergent monomers solubilize membrane proteins by partitioning into the membrane bilayer. With increasing amounts of detergents, membranes undergo various stages of solubilization. The initial stage is lysis or rupture of the membrane. At detergent:membrane lipid molar ratios of 0.1:1 through 1:1 the lipid bilayer usually remains intact but selective extraction of some membrane proteins occurs. Increasing the ratio to 2:1, solubilization of the membrane occurs resulting in mixed micelles. These include phospholipid-detergent micelles, detergent-protein micelles, and lipid-detergent-protein micelles. At a ratio of 10:1, all native membrane lipid:protein interactions are effectively exchanged for detergent:protein interactions |
7樓2014-10-23 10:18:14
z631535213
木蟲 (正式寫手)
- 應(yīng)助: 9 (幼兒園)
- 金幣: 2458.4
- 紅花: 3
- 帖子: 479
- 在線: 113.5小時(shí)
- 蟲號(hào): 2756319
- 注冊(cè): 2013-10-27
- 性別: GG
- 專業(yè): 基因組學(xué)
8樓2014-10-23 12:43:54
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 z631535213 的主題更新
| 8 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 335求調(diào)劑 +3 | yuyu宇 2026-03-23 | 4/200 |
|
|---|---|---|---|---|
|
[考研] 生物學(xué)一志愿985，分?jǐn)?shù)349求調(diào)劑 +6 | zxts12 2026-03-21 | 9/450 |
|
|
|
[考研] 341求調(diào)劑(一志愿湖南大學(xué)070300) +3 | 番茄頭--- 2026-03-22 | 4/200 |
|
|
|
[考研] 一志愿重慶大學(xué)085700資源與環(huán)境，總分308求調(diào)劑 +6 | 墨墨漠 2026-03-23 | 7/350 |
|
|
|
[考研] 08工學(xué)調(diào)劑 +7 | 用戶573181 2026-03-20 | 11/550 |
|
|
|
[考研] 263求調(diào)劑 +6 | yqdszhdap－ 2026-03-22 | 9/450 |
|
|
|
[考研] 307求調(diào)劑 +3 | 余意卿 2026-03-21 | 3/150 |
|
|
|
[考研] 一志愿中南大學(xué)化學(xué)學(xué)碩0703總分337求調(diào)劑 +4 | niko- 2026-03-22 | 4/200 |
|
|
|
[考研] 求調(diào)劑院校信息 +6 | CX 330 2026-03-21 | 6/300 |
|
|
|
[考研] 0856材料專碩353求調(diào)劑 +4 | NIFFFfff 2026-03-20 | 4/200 |
|
|
|
[考研] 材料與化工（0856）304求B區(qū)調(diào)劑 +3 | 邱gl 2026-03-20 | 7/350 |
|
|
|
[考研] 0805 316求調(diào)劑 +3 | 大雪深藏 2026-03-18 | 3/150 |
|
|
|
[考研] 22 350 本科985求調(diào)劑，求老登收留 +3 | 李軼男003 2026-03-20 | 3/150 |
|
|
|
[考研] 求調(diào)劑 +3 | 白QF 2026-03-21 | 3/150 |
|
|
|
[考研] 070300化學(xué)319求調(diào)劑 +7 | 錦鯉0909 2026-03-17 | 7/350 |
|
|
|
[考研] 308求調(diào)劑 +3 | 阿姐阿姐家啊 2026-03-18 | 3/150 |
|
|
|
[考研] 材料學(xué)碩297已過四六級(jí)求調(diào)劑推薦 +11 | adaie 2026-03-19 | 11/550 |
|
|
|
[考研] 295材料求調(diào)劑，一志愿武漢理工085601專碩 +5 | Charlieyq 2026-03-19 | 5/250 |
|
|
|
[考研] 廣西大學(xué)家禽遺傳育種課題組2026年碩士招生（接收計(jì)算機(jī)專業(yè)調(diào)劑） +3 | 123阿標(biāo) 2026-03-17 | 3/150 |
|
|
|
[碩博家園] 湖北工業(yè)大學(xué) 生命科學(xué)與健康學(xué)院-課題組招收2026級(jí)食品/生物方向碩士 +3 | 1喜春8 2026-03-17 | 5/250 |
|