 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
小木蟲論壇-學術科研互動平臺 » 生物醫(yī)藥區(qū) » 分子生物 » 核酸提取 » 關于細胞裂解的一點問題
| 8 | 1/1 | 返回列表 |
| 查看: 2757 | 回復: 7 | ||
z631535213木蟲 (正式寫手)
|
[求助]
關于細胞裂解的一點問題 已有3人參與
|
|
準備自己配點裂解液用用，看了些東西，發(fā)現(xiàn)一些裂解液中提到有效裂解成分，我目前只看到三個，Triton X-100，NP40，SDS。 想問下對這方面有了解的蟲友 1.曲通，NP40，SDS一般多少濃度可有效裂解細胞。主要是用來裂解細胞提取DNA來進行后面的實驗 2.曲通，NP40，SDS對細胞的裂解作用能具體講一講么。 恩，大概就這些。拜謝  |
回復此樓» 猜你喜歡
不合理蛙科研實驗中的趣事：實驗器材的 “烏龍”
已經(jīng)有0人回復
-
不合理蛙科研實驗中的趣事：和實驗材料的 “斗智斗勇”
已經(jīng)有0人回復
-
化學工程及工業(yè)化學論文潤色/翻譯怎么收費?
已經(jīng)有53人回復
-
蛋白質(zhì)檢測：精準分析，解鎖生物分子的密碼
已經(jīng)有0人回復
-
不合理蛙科研實驗之小鼠實驗：嚴謹設計，解析生命機制的重要載體
已經(jīng)有0人回復
-
不合理蛙科研實驗之重金屬檢測：精準篩查，守護健康與環(huán)境的防線
已經(jīng)有0人回復
-
不合理蛙科研實驗之“蛙測重金屬我背鍋三千”
已經(jīng)有0人回復
-
不合理蛙科研實驗之“鼠逃三次我發(fā)三篇SCI”
已經(jīng)有0人回復
-
納米粒度分析
已經(jīng)有3人回復
-
林科院林化所招收材料與化工專業(yè)碩士，坐標南京
已經(jīng)有0人回復
» 本主題相關價值貼推薦，對您同樣有幫助:
- 有木有誰建立細胞株的呀，求經(jīng)驗
已經(jīng)有16人回復
- 動物組織研磨和裂解問題
已經(jīng)有13人回復
- 關于293T細胞的問題，急求助
已經(jīng)有43人回復
- 170kDa膜蛋白MRP1跑WB遇到問題
已經(jīng)有39人回復
- ALP活性檢測中細胞裂解問題
已經(jīng)有10人回復
- 關于懸浮細胞MTT的一點疑問，求解答！
已經(jīng)有11人回復
- 求助：在不破壞組蛋白結(jié)構(gòu)的前提下，應該采用什么樣的細胞裂解方法？
已經(jīng)有12人回復
- 求助怎樣使細菌細胞裂解但胞內(nèi)的蛋白（例如酶）不失活？
已經(jīng)有9人回復
- 細胞裂解提取細胞內(nèi)的總蛋白，需要使用什么試劑盒，求推薦
已經(jīng)有14人回復
- 細胞裂解緩沖液的配制？求助各位大蝦
已經(jīng)有3人回復
- 關于THP-1細胞的培養(yǎng)，分化問題
已經(jīng)有12人回復
- 關于源內(nèi)裂解的問題
已經(jīng)有3人回復
- 關于質(zhì)譜裂解規(guī)律的問題
已經(jīng)有6人回復
- 求助裂解細胞又不降低酶活
已經(jīng)有5人回復
- 【求助/交流】ALP的定量檢測一些問題
已經(jīng)有6人回復
- 【求助/交流】請各位幫幫忙~~細胞裂解液如何保存，能保存多長時間？
已經(jīng)有8人回復
- 【求助/交流】為什么條帶很細啊？郁悶呢！
已經(jīng)有9人回復
- 【求助/交流】細胞裂解的問題
已經(jīng)有19人回復
- 【求助】關于裂解氣質(zhì)聯(lián)用分析的問題
已經(jīng)有4人回復
z631535213
木蟲 (正式寫手)
- 應助: 9 (幼兒園)
- 金幣: 2458.4
- 紅花: 3
- 帖子: 479
- 在線: 113.5小時
- 蟲號: 2756319
- 注冊: 2013-10-27
- 性別: GG
- 專業(yè): 基因組學
2樓2014-10-17 08:28:43
jurkat.1640
至尊木蟲 (文壇精英)
- MolEPI: 1
- 應助: 908 (博后)
- 金幣: 17168.2
- 散金: 1654
- 紅花: 121
- 沙發(fā): 41
- 帖子: 14522
- 在線: 2184.3小時
- 蟲號: 514340
- 注冊: 2008-02-28
- 性別: GG
- 專業(yè): 病毒學
3樓2014-10-17 08:58:00
z631535213
木蟲 (正式寫手)
- 應助: 9 (幼兒園)
- 金幣: 2458.4
- 紅花: 3
- 帖子: 479
- 在線: 113.5小時
- 蟲號: 2756319
- 注冊: 2013-10-27
- 性別: GG
- 專業(yè): 基因組學
4樓2014-10-17 10:31:23
5樓2014-10-22 21:34:59
6樓2014-10-23 10:03:54
【答案】應助回帖
|
Detergents are amphipathic molecules, meaning they contain both a nonpolar "tail" having aliphatic or aromatic character and a polar "head". Ionic character of the polar head group forms the basis for broad classification of detergents; they may be ionic (charged, either anionic or cationic), nonionic (uncharged) or zwitterionic (having both positively and negatively charged groups but with a net charge of zero). Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein:protein interaction. Non-denaturing detergents can be divided into nonionic detergents such as Triton* X-100, bile salts such as cholate and zwitterionic detergents such as CHAPS. A major factor determining the behavior and interaction of molecules in biological samples is their hydrophilicity or hydrophobicity. Most proteins and other molecules with charged or polar functional groups are soluble (or miscible) in water because they participate in the highly ordered, hydrogen-bonded intermolecular structure of water. Some other proteins (or at least parts of proteins), as well as fats and lipids, lack polar or charged functional groups; consequently, they are excluded from the ordered interaction of water with other polar molecules and tend to associate together in structures having minimal surface area contact with the polar environment. This association of nonpolar molecules in aqueous solutions is commonly called hydrophobic attraction. Generally, moderate concentrations of mild (i.e., nonionic) detergents compromise the integrity of cell membranes, thereby facilitating lysis of cells and extraction of soluble protein, often in native form. Using certain buffer conditions, various detergents effectively penetrate between the membrane bilayers at concentrations sufficient to form mixed micelles with isolated phospholipids and membrane proteins. Detergent monomers solubilize membrane proteins by partitioning into the membrane bilayer. With increasing amounts of detergents, membranes undergo various stages of solubilization. The initial stage is lysis or rupture of the membrane. At detergent:membrane lipid molar ratios of 0.1:1 through 1:1 the lipid bilayer usually remains intact but selective extraction of some membrane proteins occurs. Increasing the ratio to 2:1, solubilization of the membrane occurs resulting in mixed micelles. These include phospholipid-detergent micelles, detergent-protein micelles, and lipid-detergent-protein micelles. At a ratio of 10:1, all native membrane lipid:protein interactions are effectively exchanged for detergent:protein interactions |
7樓2014-10-23 10:18:14
z631535213
木蟲 (正式寫手)
- 應助: 9 (幼兒園)
- 金幣: 2458.4
- 紅花: 3
- 帖子: 479
- 在線: 113.5小時
- 蟲號: 2756319
- 注冊: 2013-10-27
- 性別: GG
- 專業(yè): 基因組學
8樓2014-10-23 12:43:54
相關版塊跳轉(zhuǎn)
我要訂閱樓主 z631535213 的主題更新
| 8 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 081700 調(diào)劑 267分 +7 | 迷人的哈哈 2026-03-23 | 7/350 |
|
|---|---|---|---|---|
|
[考研] 335分 | 材料與化工專碩 | GPA 4.07 | 有科研經(jīng)歷 +4 | cccchenso 2026-03-23 | 4/200 |
|
|
|
[考研] 一志愿中南大學化學學碩0703總分337求調(diào)劑 +5 | niko- 2026-03-22 | 5/250 |
|
|
|
[考研] 一志愿南航材料專317分求調(diào)劑 +3 | 炸呀炸呀炸薯條 2026-03-23 | 3/150 |
|
|
|
[考研] 336求調(diào)劑 +4 | 收到VS 2026-03-20 | 4/200 |
|
|
|
[考研] 上海電力大學材料防護與新材料重點實驗室招收調(diào)劑研究生（材料、化學、電化學，環(huán)境） +3 | 我愛學電池 2026-03-23 | 3/150 |
|
|
|
[考研] 298求調(diào)劑 +8 | 上岸6666@ 2026-03-20 | 8/400 |
|
|
|
[考研] 333求調(diào)劑 +6 | 87639 2026-03-21 | 10/500 |
|
|
|
[考研] 尋找調(diào)劑 +4 | 倔強芒? 2026-03-21 | 4/200 |
|
|
|
[考研] 0703化學297求調(diào)劑 +3 | Daisy☆ 2026-03-20 | 3/150 |
|
|
|
[考研] 296求調(diào)劑 +4 | www_q 2026-03-20 | 4/200 |
|
|
|
[考研] 一志愿重慶大學085700資源與環(huán)境總分308求調(diào)劑 +7 | 墨墨漠 2026-03-20 | 7/350 |
|
|
|
[考研] 268求調(diào)劑 +9 | 簡單點0 2026-03-17 | 9/450 |
|
|
|
[考研] 265求調(diào)劑 +3 | Jack?k?y 2026-03-17 | 3/150 |
|
|
|
[考研] 材料工程（專）一志愿985 初試335求調(diào)劑 +3 | hiloiy 2026-03-17 | 4/200 |
|
|
|
[考研] 一志愿華南師大 070300（化學）304分求調(diào)劑 +3 | 0703武芊慧雪304 2026-03-18 | 3/150 |
|
|
|
[考研] 一志愿武漢理工材料工程專碩調(diào)劑 +9 | Doleres 2026-03-19 | 9/450 |
|
|
|
[考研] 290求調(diào)劑 +7 | ^O^乜 2026-03-19 | 7/350 |
|
|
|
[考研] 求調(diào)劑一志愿南京航空航天大學289分 +3 | @taotao 2026-03-19 | 3/150 |
|
|
|
[考研] 材料學碩318求調(diào)劑 +5 | February_Feb 2026-03-19 | 5/250 |
|