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轉(zhuǎn)身丶忘不掉管理員
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Several polymers have been used to fabricate porous scaffolds for three-dimensional cell or tissue culture to repair bone defect,including poly (L-lactic-co-glycolic acid)(PLGA). It is one of the most widely used biodegradable polymers because of its good biocompatibility and regulable degradation rate which can be readily modulated by varying the copolymer ratio of lactic to glycolic acid [1]. Several methods have been adopted to fabricate porous biodegradable PLGA scaffold, including solvent casting/ particulate leaching [2,3], phase separation [4,5], emulsion freezedrying[6], gas foaming [7,8], electrospun [9], 3-D printing [10],fiber bonding [11] and microspheres sintering [12–15]. An ideal scaffold is supposed to bear the characteristics of excellent biocompatibility, cytocompatibility, controllable biodegradability,suitable microstructure (pore size and porosity) and mechanical properties [16,17]. Poly (b-hydroxybutyrate-co-b-hydroxyvalerate) (PHBV) copolymers,especially PHBV with 8% HV component have drawn many attention, owing to its low cytotoxicity and cell compatibility [18].Chen et al. have studied the interactions between PHBV and human keratinocytes [19], allogeneic chondrocytes [20], glial cells [21],fibroblast and osteoblast [22], and these studies proved that PHBV can support the above cells to adhere and proliferate. In addition,PHBV matrices have been proved to support fibroblast proliferation while maintaining the structural integrity [23]. When PHBV was used as sutures, it did not bring about acute inflammation, necrosis or malignant tumor within 1 year [24,25]. |
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轉(zhuǎn)身丶忘不掉: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案, 棒！ 2014-11-21 11:12:54
轉(zhuǎn)身丶忘不掉: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案, 棒！ 2014-11-21 11:12:54
| 多種高分子聚物包括聚Ｌ－乳酸－聚羥基乙酸共聚物（ＰＬＧＡ）已被用來(lái)制備多孔支架，這些支架可用作三維的細(xì)胞或組織培養(yǎng)以修復(fù)骨缺損。由于其良好的生物相容性和可調(diào)節(jié)的降解率（通過(guò)改變?nèi)樗岷鸵掖妓岬墓簿郾日{(diào)節(jié)），ＰＬＧＡ是用的最廣泛的生物可降解高聚物之一。目前已多種方法來(lái)制備多孔可生物降解的ＰＬＧＡ支架，包括溶劑澆鑄／粒子洗出。相分離、乳液冷凍干燥、氣體發(fā)泡、電紡、３－Ｄ打印、纖維成鍵以及微球燒結(jié)。理想的支架應(yīng)當(dāng)具有優(yōu)良的生物相容性、細(xì)胞相容性、可控的生物可降解性、合適的微結(jié)構(gòu)（孔徑和多孔性）以及機(jī)械性能等特點(diǎn)。羥基丁酸——羥基戊酸共聚酯（ＰＨＢＶ），特別是含有８％ＨＶ成分的ＰＨＢＶ，由于其較低的細(xì)胞毒性和細(xì)胞相容性，已廣受關(guān)注。Ｃｈｅｎ等研究了ＰＨＢＶ和人角質(zhì)形成細(xì)胞、同種異體軟骨細(xì)胞、神經(jīng)膠質(zhì)細(xì)胞、纖維組織母細(xì)胞、早骨細(xì)胞等之間的相互作用，這些研究證明了ＰＨＢＶ能支撐上述細(xì)胞以附著和增殖。此外，ＰＨＢＶ基質(zhì)已被證明在支撐成纖維細(xì)胞增殖的同時(shí)保持其結(jié)構(gòu)完整性。當(dāng)ＰＨＢＶ用作縫合時(shí)，它在一年內(nèi)不會(huì)產(chǎn)生急性發(fā)炎、壞疽或惡性瘤。 |
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