 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 2 | 1/1 | 返回列表 |
| 查看: 457 | 回復: 1 | ||
| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | ||
轉(zhuǎn)身丶忘不掉銅蟲 (小有名氣)
|
[求助]
英語論文翻譯，求助！
|
|
|
2.9. Cell culture Human mesenchymal stem cells (hMSCs) were a kind gift donated by the Second Affiliated Hospital of Sun Yat-Sen University and were propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) with supplements of 1.5 mg$ml-1 sodium bicarbonate,4.5 mg ml-1 glucose, 10% (v/v) fetal bovine serum (FBS), 3 mg ml-1 4-(2- hydroxyerhyl)piperazine-1-erhanesulfonic acid (HEPES). The cells were kept in a humidified incubator at 37℃and5%CO2, and themediumwas changed every 3 days. 2.10. Cell seeding on the scaffolds PLGA/PHBV scaffolds were cut into disks of 6mmin diameter and 2mmin height and sterilized by exposing to gamma radiation (15 kGy). The pure PLGA scaffolds of the same size as that of PLGA/PHBV were immersed in 75% (v/v) ethanol aqueous solution for 2 h and followed by ultraviolet radiation for 30 min to sterilize. All the scaffolds were prewet in the DMEM solution for 24 h. Fifteen microlitres of passage 6 cells in suspension (7.5×104 cells/well) were seeded on each scaffold. 2 h later, 700 μl of culture medium was added into each well. The cells/scaffold constructs were incubated at 37℃ in a humidified incubator of 5% CO2 for pre-set days. |
回復此樓» 猜你喜歡
26考研一志愿中國石油大學(華東)305分求調(diào)劑
已經(jīng)有4人回復
-
286求調(diào)劑
已經(jīng)有5人回復
-
材料與化工（0856）304求 B區(qū) 調(diào)劑
已經(jīng)有3人回復
-
268求調(diào)劑
已經(jīng)有8人回復
-
265求調(diào)劑
已經(jīng)有14人回復
-
350 本科985求調(diào)劑，求老登收留
已經(jīng)有3人回復
-
【考研調(diào)劑】化學專業(yè) 281分，一志愿四川大學，誠心求調(diào)劑
已經(jīng)有9人回復
-
初試 317
已經(jīng)有3人回復
-
279分求調(diào)劑 一志愿211
已經(jīng)有15人回復
-
279求調(diào)劑
已經(jīng)有4人回復
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
轉(zhuǎn)身丶忘不掉: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案 2014-12-07 13:41:10
轉(zhuǎn)身丶忘不掉: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案 2014-12-07 13:41:10
|
細胞培養(yǎng) 人類間充質(zhì)干細胞(hMSCs)是一種禮物由中山大學第二附屬醫(yī)院的捐贈和在Dulbecco修改鷹的傳播媒介(DMEM)補充1.5毫克ml-1美元碳酸氫鈉,4.5毫克ml-1葡萄糖,10%(v / v)胎牛血清的邊后衛(wèi),3毫克ml-1 4 -(2 - hydroxyerhyl)piperazine-1-erhanesulfonic酸(玫瑰)。這些細胞被保存在一個濕潤孵化器在37℃and5%CO2,和themediumwas每3天換一次。 2.10。細胞支架上播種 PLGA / PHBV支架被切成磁盤6 mmin直徑和2 mmin高度和消毒暴露伽馬輻射(15 kGy的)。相同大小的純PLGA支架的PLGA / PHBV沉浸在75%(v / v)乙醇水溶液為2 h,緊隨其后的是紫外線消毒30分鐘。所有的支架都是兩高一低的DMEM 24 h。15毫升通道解決方案6細胞懸浮(7.5×104細胞/)被播種在每個支架。2 h后,700μl培養(yǎng)基添加到每個。細胞/支架結(jié)構(gòu)在37℃濕潤孵化器孵化預設(shè)天5%的二氧化碳。 |
2樓2014-12-05 15:47:21
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 轉(zhuǎn)身丶忘不掉 的主題更新
| 2 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 求調(diào)劑 +3 | 白QF 2026-03-21 | 3/150 |
|
|---|---|---|---|---|
|
|
[考研] 330求調(diào)劑0854 +3 | assdll 2026-03-21 | 3/150 |
|
|
|
[考研] 材料 271求調(diào)劑 +3 | 展信悅_ 2026-03-21 | 3/150 |
|
|
|
[考研] 316求調(diào)劑 +6 | 梁茜雯 2026-03-19 | 6/300 |
|
|
|
[考研] 070300化學319求調(diào)劑 +7 | 錦鯉0909 2026-03-17 | 7/350 |
|
|
|
[考研] 307求調(diào)劑 +3 | wyyyqx 2026-03-17 | 3/150 |
|
|
|
[考研] 303求調(diào)劑 +5 | 睿08 2026-03-17 | 7/350 |
|
|
[考研] 二本跨考鄭大材料306英一數(shù)二 +3 | z1z2z3879 2026-03-17 | 3/150 |
|
|
|
[考研] 一志愿中海洋材料工程專碩330分求調(diào)劑 +8 | 小材化本科 2026-03-18 | 8/400 |
|
|
|
[考研] 304求調(diào)劑 +7 | 司空. 2026-03-18 | 7/350 |
|
|
|
[考研] 288求調(diào)劑 +16 | 于海海海海 2026-03-19 | 16/800 |
|
|
|
[考研] 317求調(diào)劑 +5 | 申子申申 2026-03-19 | 9/450 |
|
|
|
[考研] 085600材料與化工 +8 | 安全上岸！ 2026-03-16 | 8/400 |
|
|
|
[考研] 一志愿中南化學（0703）總分337求調(diào)劑 +8 | niko- 2026-03-19 | 9/450 |
|
|
|
[考研] 289求調(diào)劑 +6 | 懷瑾握瑜l 2026-03-20 | 6/300 |
|
|
|
[考研] 【同濟軟件】軟件（085405）考研求調(diào)劑 +3 | 2026eternal 2026-03-18 | 3/150 |
|
|
|
[考研] 考研求調(diào)劑 +3 | 橘頌. 2026-03-17 | 4/200 |
|
|
|
[考研] 301求調(diào)劑 +4 | A_JiXing 2026-03-16 | 4/200 |
|
|
|
[考研] 材料專碩326求調(diào)劑 +6 | 墨煜姒莘 2026-03-15 | 7/350 |
|
|
|
[考研] [導師推薦]西南科技大學國防/材料導師推薦 +3 | 尖角小荷 2026-03-16 | 6/300 |
|