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轉(zhuǎn)身丶忘不掉銅蟲(chóng) (小有名氣)
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[求助]
英語(yǔ)論文翻譯，求助！
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2.9. Cell culture Human mesenchymal stem cells (hMSCs) were a kind gift donated by the Second Affiliated Hospital of Sun Yat-Sen University and were propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) with supplements of 1.5 mg$ml-1 sodium bicarbonate,4.5 mg ml-1 glucose, 10% (v/v) fetal bovine serum (FBS), 3 mg ml-1 4-(2- hydroxyerhyl)piperazine-1-erhanesulfonic acid (HEPES). The cells were kept in a humidified incubator at 37℃and5%CO2, and themediumwas changed every 3 days. 2.10. Cell seeding on the scaffolds PLGA/PHBV scaffolds were cut into disks of 6mmin diameter and 2mmin height and sterilized by exposing to gamma radiation (15 kGy). The pure PLGA scaffolds of the same size as that of PLGA/PHBV were immersed in 75% (v/v) ethanol aqueous solution for 2 h and followed by ultraviolet radiation for 30 min to sterilize. All the scaffolds were prewet in the DMEM solution for 24 h. Fifteen microlitres of passage 6 cells in suspension (7.5×104 cells/well) were seeded on each scaffold. 2 h later, 700 μl of culture medium was added into each well. The cells/scaffold constructs were incubated at 37℃ in a humidified incubator of 5% CO2 for pre-set days. |
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
轉(zhuǎn)身丶忘不掉: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案 2014-12-07 13:41:10
轉(zhuǎn)身丶忘不掉: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案 2014-12-07 13:41:10
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細(xì)胞培養(yǎng) 人類間充質(zhì)干細(xì)胞(hMSCs)是一種禮物由中山大學(xué)第二附屬醫(yī)院的捐贈(zèng)和在Dulbecco修改鷹的傳播媒介(DMEM)補(bǔ)充1.5毫克ml-1美元碳酸氫鈉,4.5毫克ml-1葡萄糖,10%(v / v)胎牛血清的邊后衛(wèi),3毫克ml-1 4 -(2 - hydroxyerhyl)piperazine-1-erhanesulfonic酸(玫瑰)。這些細(xì)胞被保存在一個(gè)濕潤(rùn)孵化器在37℃and5%CO2,和themediumwas每3天換一次。 2.10。細(xì)胞支架上播種 PLGA / PHBV支架被切成磁盤(pán)6 mmin直徑和2 mmin高度和消毒暴露伽馬輻射(15 kGy的)。相同大小的純PLGA支架的PLGA / PHBV沉浸在75%(v / v)乙醇水溶液為2 h,緊隨其后的是紫外線消毒30分鐘。所有的支架都是兩高一低的DMEM 24 h。15毫升通道解決方案6細(xì)胞懸浮(7.5×104細(xì)胞/)被播種在每個(gè)支架。2 h后,700μl培養(yǎng)基添加到每個(gè)。細(xì)胞/支架結(jié)構(gòu)在37℃濕潤(rùn)孵化器孵化預(yù)設(shè)天5%的二氧化碳。 |
2樓2014-12-05 15:47:21
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