 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 3 | 1/1 | 返回列表 |
| 查看: 780 | 回復(fù): 2 | ||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
huojinlong8610金蟲 (小有名氣)
|
[求助]
潤色英文摘要
|
|
|
摘 要：白細(xì)胞介素6（IL-6）是機(jī)體重要的免疫調(diào)節(jié)因子，在佐劑的應(yīng)用方面具有很好的發(fā)展前景。本研究以版納微型豬近交系（BMI）為實(shí)驗(yàn)動(dòng)物，通過RT-PCR法獲得IL-6基因編碼區(qū)序列，提交GenBank數(shù)據(jù)庫，登錄號(hào)為“JQ839263”。對(duì)BMI IL-6基因和相應(yīng)的蛋白質(zhì)序列進(jìn)行了多重生物信息學(xué)分析，結(jié)果顯示該基因編碼212個(gè)氨基酸，蛋白分子量（Mw）為23.88 kD，等電點(diǎn)（pI）為6.66，存在一個(gè)保守結(jié)構(gòu)域，一個(gè)跨膜結(jié)構(gòu)，N端和C端均疏水，且在N端存在信號(hào)肽，有89%的可能性定位于細(xì)胞核。將BMI的IL-6基因編碼區(qū)序列與NCBI下載到的4條豬IL-6基因序列進(jìn)行比對(duì)結(jié)果顯示BMI IL6與NM_214399和DQ832259核酸序列完全相同，與AF309651和AF518322各存在1處堿基差異；多物種氨基酸序列比對(duì)及系統(tǒng)進(jìn)化分析表明，BMI與駱駝?dòng)H緣關(guān)系最近。同時(shí)利用半定量PCR法確定了IL-6基因mRNA在BMI 12個(gè)組織中的表達(dá)量，發(fā)現(xiàn)在肺、皮膚、肌肉中表達(dá)量較高。本研究為進(jìn)一步研究豬IL-6基因的表達(dá)調(diào)控奠定了理論基礎(chǔ)。 Abstract：The interleukin-6 (IL-6) is an important immune regulatory factor, and has great development prospects in the application of adjuvants. Using the Banna Mini-pig Inbred Line (BMI) as the experimental animal, we successfully cloned the coding region of porcine IL-6 gene through the reverse transcriptase polymerase chain reaction (RT-PCR) method, and had deposited the sequence into NCBI database and assigned to the accession No.: JQ839263. Then we further study this gene by multiple bioinformatics analyses method. Results showed that BMI IL-6 encoded 212 amino acids with a predicted molecular weight of 23.88 kD and the isoelectric point (pI) 6.66 and a signal peptide in N-terminal. It is a nuclear protein with a 89% probability. In addition, BMI IL-6 contained one conserved domain and its N-terminal and C-terminal were both hydrophobic. Comparing the sequence of the coding region of BMI IL-6 gene with the download 4 pig IL-6 gene sequences, the result shows BMI is identical with NM_214399 and DQ832259, only had 1 base pair difference with AF309651 and AF518322 respectively. Multi-species amino acid sequence alignment and phylogenetic analysis showed that BMI has a closest relationship with camel. Finally, we analysed IL-6 gene mRNA expression in 12 important tissues of BMI through RT-PCR method, results showed that BMI IL-6 gene was highly expressed in lung, skin and muscle of BMI adult pig. These data serve as a foundation for further insight into the expression and regulation of this porcine gene. |
回復(fù)此樓» 猜你喜歡
本人考085602 化學(xué)工程 專碩
已經(jīng)有23人回復(fù)
-
求調(diào)劑院校信息
已經(jīng)有4人回復(fù)
-
085600材料與化工306
已經(jīng)有4人回復(fù)
-
286求調(diào)劑
已經(jīng)有10人回復(fù)
-
328求調(diào)劑，英語六級(jí)551，有科研經(jīng)歷
已經(jīng)有9人回復(fù)
-
一志愿北京化工大學(xué)070300 學(xué)碩336求調(diào)劑
已經(jīng)有4人回復(fù)
-
286分人工智能專業(yè)請求調(diào)劑愿意跨考！
已經(jīng)有8人回復(fù)
-
資源與環(huán)境 調(diào)劑申請(333分)
已經(jīng)有5人回復(fù)
-
280求調(diào)劑
已經(jīng)有12人回復(fù)
-
269專碩求調(diào)劑
已經(jīng)有5人回復(fù)
ssssllllnnnn 
至尊木蟲 (知名作家)
Translator and Proofreader
- 翻譯EPI: 1690
- 應(yīng)助: 452 (碩士)
- 金幣: 31580.9
- 紅花: 100
- 帖子: 7681
- 在線: 19966.6小時(shí)
- 蟲號(hào): 3328089
- 注冊: 2014-07-17
- 專業(yè): 腫瘤發(fā)生
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
RXMCDM: 金幣+2, 多謝應(yīng)助！ 2014-12-01 08:23:32
huojinlong8610: 金幣+80, 翻譯EPI+1, ★★★很有幫助 2014-12-02 21:55:39
RXMCDM: 金幣+2, 多謝應(yīng)助！ 2014-12-01 08:23:32
huojinlong8610: 金幣+80, 翻譯EPI+1, ★★★很有幫助 2014-12-02 21:55:39
|
中文部分也需要潤色： 1、通過RT-PCR法獲得IL-6基因編碼區(qū)序列：通過RT-PCR法獲得IL-6 mRNA編碼區(qū)序列（不能用基因代替mRNA，反之亦然）； 2、存在一個(gè)保守結(jié)構(gòu)域：這個(gè)描述缺乏具體性，應(yīng)該具體指出該結(jié)構(gòu)域的名字; 3、多物種氨基酸序列比對(duì)及系統(tǒng)進(jìn)化分析表明，BMI與駱駝?dòng)H緣關(guān)系最近：多物種氨基酸序列比對(duì)及系統(tǒng)進(jìn)化分析表明，就IL-6而言BMI與駱駝?dòng)H緣關(guān)系最近（讀你的原話會(huì)誤以為BMI和駱駝?dòng)H緣關(guān)系最近�。� 4、同時(shí)利用半定量PCR法確定了IL-6基因mRNA：同時(shí)利用半定量RT-PCR法確定了IL-6基因mRNA（RT-PCR不能與PCR混稱）。 5、提交GenBank數(shù)據(jù)庫，登錄號(hào)為“JQ839263”：此內(nèi)容無需寫于摘要中（可放腳注） 另外：”有89%的可能性定位于細(xì)胞核“，此結(jié)論在文中有什么意義？眾所周知IL-6是一種分泌蛋白（所以有信號(hào)肽），IL-6難道也定位于細(xì)胞核嗎（我未去確證，但感覺即便有也不是主要的）。 英文部分： Abstract：Interleukin-6 (IL-6) 【無需用The】 is an important immune regulatory factor, and has great development prospects in the application of adjuvants. Using 【此處的the也要去掉】 Banna Mini-pig Inbred Line (BMI) as the experimental animalS【此處最好用復(fù)數(shù)】, we successfully cloned the coding region of porcine IL-6 mRNA 【用mRNA而非gene，你并非克隆了基因】 through the 【此the可去掉】 reverse transcriptase polymerase chain reaction (RT-PCR) method【無需用method】, and had 【摘要中不需要用過去完成時(shí)，去掉had】 deposited the sequence into NCBI database and assigned to the accession No.: JQ839263. Then we further study 【時(shí)態(tài)統(tǒng)一，用過去時(shí)studied】this gene by multiple bioinformatics analyses method【不需要用method，多余】. Results showed that BMI IL-6 encoded 【這里該用一般現(xiàn)在時(shí)encode，描述結(jié)論時(shí)用現(xiàn)在時(shí)】 212 amino acids with a predicted molecular weight of 23.88 kD and【將and換為逗號(hào)，】 the isoelectric point (pI) 6.66 and a signal peptide in【at】 N-terminal【terminus】. It is a nuclear protein with a 89% probability【建議省去次句話，所以就不改英文說法了】. In addition, BMI IL-6 contains【用現(xiàn)在時(shí)】 one conserved domain and both its N- and C-termini 【此處修改了，注意對(duì)照】are 【現(xiàn)在時(shí)】 hydrophobic. Comparing the sequence of the coding region of BMI IL-6 mRNA 【仍然不能用gene替代mRNA】 with the download 【該用downloaded，其實(shí)該詞多余】 four 【不用阿拉伯?dāng)?shù)字】 pig IL-6 gene【該用mRNA】 sequences, the【去掉此the】 result shows 【加上：that the sequence of】 BMI is identical with NM_214399 and DQ832259, only has【用現(xiàn)在時(shí)】 one【不用阿拉伯?dāng)?shù)字】 base pair difference with AF309651 and AF518322 respectively. Multi-species amino acid sequence alignment and phylogenetic analysis showed that 【加上：IL-6 of】BMI 【最好加上origin】has a【改用the】 closest relationship with 【加上that of】 camel. Finally, we analysed IL-6 gene【此gene去掉】 mRNA expression in 12 important tissues of BMI through 【加上semi-quantitative】 RT-PCR method【此處的method可去掉】, results showed that BMI IL-6 gene 【用mRNA替代gene】 was highly expressed in lung, skin and muscle of BMI adult pig. These data lay the foundation【此處做了修改】 for further studies of the expression and regulation of this porcine gene. 草草修改了一下，僅供參考 |
2樓2014-11-30 22:05:52
mac194
鐵蟲 (職業(yè)作家)
- 翻譯EPI: 61
- 應(yīng)助: 128 (高中生)
- 金幣: 21401.3
- 紅花: 101
- 帖子: 3332
- 在線: 3370.2小時(shí)
- 蟲號(hào): 2488597
- 注冊: 2013-05-30
- 專業(yè): 高分子材料的加工與成型
【答案】應(yīng)助回帖
|
這不是摘要,所有重點(diǎn)句的總和不一定就是摘要 除非實(shí)驗(yàn)步驟新穎,不需贅述 請教: 你的實(shí)驗(yàn)結(jié)果和以下 2010 文章有何關(guān)聯(lián),比人家強(qiáng)多少 ??? https://www.biopharminternationa ... ticle/detail/651187 ----- 2010 相關(guān)文章.jpg |
3樓2014-12-01 11:26:20
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 huojinlong8610 的主題更新
| 3 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿北京化工大學(xué)070300 學(xué)碩336求調(diào)劑 +3 | vv迷 2026-03-21 | 4/200 |
|
|---|---|---|---|---|
|
|
[考研]
 一志愿東華大學(xué)控制學(xué)碩320求調(diào)劑
+3
|
Grand777 2026-03-21 | 3/150 |
|
|
[考研] 生物學(xué)調(diào)劑 +3 | Surekei 2026-03-21 | 3/150 |
|
|
|
[考研] 一志愿深大，0703化學(xué)，總分302，求調(diào)劑 +4 | 七月-七七 2026-03-21 | 4/200 |
|
|
|
[考研] 317求調(diào)劑 +9 | 申子申申 2026-03-19 | 15/750 |
|
|
|
[考研] 材料 271求調(diào)劑 +5 | 展信悅_ 2026-03-21 | 5/250 |
|
|
|
[考研] 336求調(diào)劑 +5 | rmc8866 2026-03-21 | 5/250 |
|
|
|
[考研] 生物學(xué)一志愿985，分?jǐn)?shù)349求調(diào)劑 +3 | zxts12 2026-03-21 | 3/150 |
|
|
|
[考研] 299求調(diào)劑 +4 | 某某某某位 2026-03-21 | 4/200 |
|
|
|
[考研] 332求調(diào)劑 +3 | 鳳凰院丁真 2026-03-20 | 3/150 |
|
|
|
[考研] 0856材料專碩353求調(diào)劑 +3 | NIFFFfff 2026-03-20 | 3/150 |
|
|
|
[考研] 能源材料化學(xué)課題組招收碩士研究生8-10名 +5 | 脫穎而出 2026-03-16 | 15/750 |
|
|
|
[考研] 08工科 320總分 求調(diào)劑 +6 | 梨花珞晚風(fēng) 2026-03-17 | 6/300 |
|
|
|
[考研] 085700資源與環(huán)境308求調(diào)劑 +12 | 墨墨漠 2026-03-18 | 13/650 |
|
|
|
[考研] 295求調(diào)劑 +4 | 一志愿京區(qū)211 2026-03-18 | 6/300 |
|
|
|
[考研] 260求調(diào)劑 +3 | 朱芷琳 2026-03-20 | 3/150 |
|
|
|
[考研] 353求調(diào)劑 +3 | 拉鉤不許變 2026-03-20 | 3/150 |
|
|
|
[考研] 一志愿985，本科211，0817化學(xué)工程與技術(shù)319求調(diào)劑 +10 | Liwangman 2026-03-15 | 10/500 |
|
|
|
[考研]
0854，計(jì)算機(jī)類招收調(diào)劑
+3
|
胡辣湯放糖 2026-03-15 | 6/300 |
|
|
|
[考研] 東南大學(xué)364求調(diào)劑 +5 | JasonYuiui 2026-03-15 | 5/250 |
|