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huojinlong8610金蟲 (小有名氣)
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[求助]
潤色英文摘要
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摘 要：白細胞介素6（IL-6）是機體重要的免疫調(diào)節(jié)因子，在佐劑的應(yīng)用方面具有很好的發(fā)展前景。本研究以版納微型豬近交系（BMI）為實驗動物，通過RT-PCR法獲得IL-6基因編碼區(qū)序列，提交GenBank數(shù)據(jù)庫，登錄號為“JQ839263”。對BMI IL-6基因和相應(yīng)的蛋白質(zhì)序列進行了多重生物信息學分析，結(jié)果顯示該基因編碼212個氨基酸，蛋白分子量（Mw）為23.88 kD，等電點（pI）為6.66，存在一個保守結(jié)構(gòu)域，一個跨膜結(jié)構(gòu)，N端和C端均疏水，且在N端存在信號肽，有89%的可能性定位于細胞核。將BMI的IL-6基因編碼區(qū)序列與NCBI下載到的4條豬IL-6基因序列進行比對結(jié)果顯示BMI IL6與NM_214399和DQ832259核酸序列完全相同，與AF309651和AF518322各存在1處堿基差異；多物種氨基酸序列比對及系統(tǒng)進化分析表明，BMI與駱駝親緣關(guān)系最近。同時利用半定量PCR法確定了IL-6基因mRNA在BMI 12個組織中的表達量，發(fā)現(xiàn)在肺、皮膚、肌肉中表達量較高。本研究為進一步研究豬IL-6基因的表達調(diào)控奠定了理論基礎(chǔ)。 Abstract：The interleukin-6 (IL-6) is an important immune regulatory factor, and has great development prospects in the application of adjuvants. Using the Banna Mini-pig Inbred Line (BMI) as the experimental animal, we successfully cloned the coding region of porcine IL-6 gene through the reverse transcriptase polymerase chain reaction (RT-PCR) method, and had deposited the sequence into NCBI database and assigned to the accession No.: JQ839263. Then we further study this gene by multiple bioinformatics analyses method. Results showed that BMI IL-6 encoded 212 amino acids with a predicted molecular weight of 23.88 kD and the isoelectric point (pI) 6.66 and a signal peptide in N-terminal. It is a nuclear protein with a 89% probability. In addition, BMI IL-6 contained one conserved domain and its N-terminal and C-terminal were both hydrophobic. Comparing the sequence of the coding region of BMI IL-6 gene with the download 4 pig IL-6 gene sequences, the result shows BMI is identical with NM_214399 and DQ832259, only had 1 base pair difference with AF309651 and AF518322 respectively. Multi-species amino acid sequence alignment and phylogenetic analysis showed that BMI has a closest relationship with camel. Finally, we analysed IL-6 gene mRNA expression in 12 important tissues of BMI through RT-PCR method, results showed that BMI IL-6 gene was highly expressed in lung, skin and muscle of BMI adult pig. These data serve as a foundation for further insight into the expression and regulation of this porcine gene. |
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★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
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huojinlong8610: 金幣+80, 翻譯EPI+1, ★★★很有幫助 2014-12-02 21:55:39
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中文部分也需要潤色： 1、通過RT-PCR法獲得IL-6基因編碼區(qū)序列：通過RT-PCR法獲得IL-6 mRNA編碼區(qū)序列（不能用基因代替mRNA，反之亦然）； 2、存在一個保守結(jié)構(gòu)域：這個描述缺乏具體性，應(yīng)該具體指出該結(jié)構(gòu)域的名字; 3、多物種氨基酸序列比對及系統(tǒng)進化分析表明，BMI與駱駝親緣關(guān)系最近：多物種氨基酸序列比對及系統(tǒng)進化分析表明，就IL-6而言BMI與駱駝親緣關(guān)系最近（讀你的原話會誤以為BMI和駱駝親緣關(guān)系最近！） 4、同時利用半定量PCR法確定了IL-6基因mRNA：同時利用半定量RT-PCR法確定了IL-6基因mRNA（RT-PCR不能與PCR混稱）。 5、提交GenBank數(shù)據(jù)庫，登錄號為“JQ839263”：此內(nèi)容無需寫于摘要中（可放腳注） 另外：”有89%的可能性定位于細胞核“，此結(jié)論在文中有什么意義？眾所周知IL-6是一種分泌蛋白（所以有信號肽），IL-6難道也定位于細胞核嗎（我未去確證，但感覺即便有也不是主要的）。 英文部分： Abstract：Interleukin-6 (IL-6) 【無需用The】 is an important immune regulatory factor, and has great development prospects in the application of adjuvants. Using 【此處的the也要去掉】 Banna Mini-pig Inbred Line (BMI) as the experimental animalS【此處最好用復(fù)數(shù)】, we successfully cloned the coding region of porcine IL-6 mRNA 【用mRNA而非gene，你并非克隆了基因】 through the 【此the可去掉】 reverse transcriptase polymerase chain reaction (RT-PCR) method【無需用method】, and had 【摘要中不需要用過去完成時，去掉had】 deposited the sequence into NCBI database and assigned to the accession No.: JQ839263. Then we further study 【時態(tài)統(tǒng)一，用過去時studied】this gene by multiple bioinformatics analyses method【不需要用method，多余】. Results showed that BMI IL-6 encoded 【這里該用一般現(xiàn)在時encode，描述結(jié)論時用現(xiàn)在時】 212 amino acids with a predicted molecular weight of 23.88 kD and【將and換為逗號，】 the isoelectric point (pI) 6.66 and a signal peptide in【at】 N-terminal【terminus】. It is a nuclear protein with a 89% probability【建議省去次句話，所以就不改英文說法了】. In addition, BMI IL-6 contains【用現(xiàn)在時】 one conserved domain and both its N- and C-termini 【此處修改了，注意對照】are 【現(xiàn)在時】 hydrophobic. Comparing the sequence of the coding region of BMI IL-6 mRNA 【仍然不能用gene替代mRNA】 with the download 【該用downloaded，其實該詞多余】 four 【不用阿拉伯數(shù)字】 pig IL-6 gene【該用mRNA】 sequences, the【去掉此the】 result shows 【加上：that the sequence of】 BMI is identical with NM_214399 and DQ832259, only has【用現(xiàn)在時】 one【不用阿拉伯數(shù)字】 base pair difference with AF309651 and AF518322 respectively. Multi-species amino acid sequence alignment and phylogenetic analysis showed that 【加上：IL-6 of】BMI 【最好加上origin】has a【改用the】 closest relationship with 【加上that of】 camel. Finally, we analysed IL-6 gene【此gene去掉】 mRNA expression in 12 important tissues of BMI through 【加上semi-quantitative】 RT-PCR method【此處的method可去掉】, results showed that BMI IL-6 gene 【用mRNA替代gene】 was highly expressed in lung, skin and muscle of BMI adult pig. These data lay the foundation【此處做了修改】 for further studies of the expression and regulation of this porcine gene. 草草修改了一下，僅供參考 |
2樓2014-11-30 22:05:52
mac194
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這不是摘要,所有重點句的總和不一定就是摘要 除非實驗步驟新穎,不需贅述 請教: 你的實驗結(jié)果和以下 2010 文章有何關(guān)聯(lián),比人家強多少 ??? https://www.biopharminternationa ... ticle/detail/651187 ----- 2010 相關(guān)文章.jpg |
3樓2014-12-01 11:26:20
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