 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 10 | 1/1 | 返回列表 |
| 查看: 1493 | 回復(fù): 9 | ||
junshane木蟲 (正式寫手)
|
[求助]
含氨基官能團(tuán)的小分子接枝改性聚丙烯酸納米纖維膜，求助，謝謝！
|
| RT，最近制備出了一種聚丙烯酸（PAA）的納米纖維膜（不溶于水），想在纖維表面用含氨基官能團(tuán)的小分子加以改性，基本上就是常規(guī)的酰胺化反應(yīng)。常見的方法是先把PAA的羧基酰氯化，再接枝氨基小分子，但是考慮這種方法會(huì)對(duì)納米纖維的形態(tài)構(gòu)成很大的影響，所以想尋求一種溫和的方法，在以水為反應(yīng)體系以及不犧牲納米纖維完整形態(tài)的前提下，完成酰胺化反應(yīng)，望各位蟲友相助，謝謝！ |
回復(fù)此樓» 猜你喜歡
一志愿北京化工大學(xué)材料與化工（085600）296求調(diào)劑
已經(jīng)有22人回復(fù)
-
322求調(diào)劑：一志愿湖南大學(xué) 材料與化工（085600），已過六級(jí)。
已經(jīng)有7人回復(fù)
-
332求調(diào)劑
已經(jīng)有5人回復(fù)
-
310求調(diào)劑
已經(jīng)有10人回復(fù)
-
26考研-291分-廈門大學(xué)（085601）-柔性電子學(xué)院材料工程專業(yè)求調(diào)劑
已經(jīng)有5人回復(fù)
-
085600 材料與化工 329分求調(diào)劑
已經(jīng)有15人回復(fù)
-
化學(xué)0703 調(diào)劑 306分 一志愿211
已經(jīng)有8人回復(fù)
-
342求調(diào)劑
已經(jīng)有4人回復(fù)
-
334分 一志愿武理 材料求調(diào)劑
已經(jīng)有14人回復(fù)
-
材料科學(xué)與工程 317求調(diào)劑
已經(jīng)有5人回復(fù)
» 本主題相關(guān)商家推薦: (我也要在這里推廣)
2樓2014-12-03 22:24:45
junshane
木蟲 (正式寫手)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 2725.5
- 散金: 1219
- 紅花: 2
- 帖子: 412
- 在線: 141.4小時(shí)
- 蟲號(hào): 1415150
- 注冊(cè): 2011-09-24
- 性別: GG
- 專業(yè): 高分子材料與環(huán)境
3樓2014-12-04 11:15:17
4樓2014-12-04 11:27:00
junshane
木蟲 (正式寫手)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 2725.5
- 散金: 1219
- 紅花: 2
- 帖子: 412
- 在線: 141.4小時(shí)
- 蟲號(hào): 1415150
- 注冊(cè): 2011-09-24
- 性別: GG
- 專業(yè): 高分子材料與環(huán)境
5樓2014-12-04 19:04:10
OD8811
至尊木蟲 (小有名氣)
- 應(yīng)助: 38 (小學(xué)生)
- 金幣: 17784.7
- 紅花: 1
- 帖子: 109
- 在線: 230.2小時(shí)
- 蟲號(hào): 1952119
- 注冊(cè): 2012-08-23
- 性別: GG
- 專業(yè): 高分子物理與高分子物理化
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
junshane: 金幣+19, ★★★很有幫助 2014-12-05 17:21:47
感謝參與，應(yīng)助指數(shù) +1
junshane: 金幣+19, ★★★很有幫助 2014-12-05 17:21:47
| 可以嘗試一下EDC coupling， https://www.piercenet.com/instructions/2160475.pdf |
6樓2014-12-04 21:48:05
junshane
木蟲 (正式寫手)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 2725.5
- 散金: 1219
- 紅花: 2
- 帖子: 412
- 在線: 141.4小時(shí)
- 蟲號(hào): 1415150
- 注冊(cè): 2011-09-24
- 性別: GG
- 專業(yè): 高分子材料與環(huán)境
7樓2014-12-05 17:23:04
OD8811
至尊木蟲 (小有名氣)
- 應(yīng)助: 38 (小學(xué)生)
- 金幣: 17784.7
- 紅花: 1
- 帖子: 109
- 在線: 230.2小時(shí)
- 蟲號(hào): 1952119
- 注冊(cè): 2012-08-23
- 性別: GG
- 專業(yè): 高分子物理與高分子物理化
|
Number Description 22980 EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride), 5g 22981 EDC, 25g 77149 EDC, 10mg Molecular Weight: 191.7 CAS # 25952-53-8 Storage: Upon receipt store Product No. 22980 and 22981 desiccated at -20°C. Store Product No. 77149 at 4°C. EDC is shipped at ambient temperature. Introduction The Thermo Scientific EDC is a carboxyl and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond and release of an isourea by-product (see the Additional Information Section). The intermediate is unstable in aqueous solutions, and therefore, two-step conjugation procedures rely on N-hydroxysuccinimide for stabilization.1,2 Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.1,3 Procedure for Using EDC for Coupling Haptens to a Carrier Protein Materials Required • Carrier protein: 2mg bovine serum albumin (BSA), ovalbumin (OVA) or keyhole limpet hemocyanin (KLH) • Conjugation Buffer: 0.1M MES (2-[N-morpholino]ethane sulfonic acid), pH 4.5-5 (Product No. 28390) • EDC: 10mg • Hapten: 1-2mg • Thermo Scientific Zeba Spin Desalting Column (Product No. 89891) or other gel filtration column with a 5-6K molecular-weight cutoff Procedure 1. Equilibrate EDC to room temperature. 2. Add 2mg of lyophilized BSA, OVA or KLH to 200μL Conjugation Buffer. If using Thermo Scientific Imject Carrier Proteins, reconstitute using ultrapure water. 3. Dissolve up to 2mg of the peptide or hapten in 500μL of Conjugation Buffer and add it to the 200μL carrier protein solution. 4. For BSA or OVA conjugation, dissolve 10mg of EDC in 1mL of ultrapure water and immediately add 100μL of this solution to the carrier-peptide solution. For KLH conjugation, dissolve 10mg of EDC in 1mL of ultrapure water and immediately add 50μL of this solution to the carrier-peptide solution. Further reduce the amount of EDC if precipitation occurs. 5. React for 2 hours at room temperature. 6. Purify the conjugate using a desalting column. If storing the immunogen for more than a few days, sterile filter the conjugate and store in a sterile container at 4°C or -20°C. Procedure for Two-step Coupling of Proteins Using EDC and NHS or Sulfo-NHS The following protocol, adapted from a procedure described by Grabarek and Gergely, allows sequential coupling of two proteins without affecting the second protein’s carboxyls by exposing them to EDC. This procedure requires quenching the first reaction with a thiol-containing compound. The activation reaction with EDC and Sulfo-NHS is most efficient at pH 4.5-7.2; however, the reaction of NHS-activated or Sulfo-NHS-activated molecules with primary amines is most efficient at pH 7-8. For best results, perform the first reaction in MES buffer (or other non-amine, non-carboxylate buffer) at pH 5-6, then raise the pH to 7.2-7.5 with phosphate buffer (or other non-amine buffer) immediately before reaction to the amine-containing molecule. For quenching the first reaction, use 2-mercaptoethanol, or the excess reagent can be simply removed (as well as the reaction pH adjusted) by buffer-exchange with a desalting column. Materials Required • Activation Buffer: 0.1M MES, 0.5M NaCl, pH 6.0 • Coupling Buffer: Phosphate-buffered saline (PBS), 100mM sodium phosphate, 150mM NaCl; pH 7.2 (Product No. 28372) • Protein # 1: Prepared in Activation Buffer at 1mg/mL • Protein # 2: Prepared in Coupling Buffer • NHS or Sulfo-NHS (Product No. 24500 and 24510, respectively) • 2-Mercaptoethanol (Product No. 35600) • (Optional) Zeba™ Spin Desalting Column (Product No. 89891) or other gel filtration column • Hydroxylamine•HCl (Product No. 26103) Procedure 1. Equilibrate EDC and NHS to room temperature before opening bottles. 2. Add 0.4mg EDC (~2mM) and 0.6mg of NHS or 1.1mg of sulfo-NHS (~5mM) to 1mL of protein #1 solution and react for 15 minutes at room temperature. 3. Add 1.4μL of 2-mercaptoethanol (final concentration of 20mM) to quench the EDC. 4. Optional: Separate the protein from excess reducing agent and inactivated crosslinker using a desalting column that has been equilibrated with Coupling Buffer (PBS). 5. Add protein #2 to the activated protein at an equal molar ratio with protein #1. Allow the proteins to react for 2 hours at room temperature. 6. To quench the reaction, add hydroxylamine to a final concentration of 10mM. This method hydrolyzes nonreacted NHS present on protein #1 and results in hydroxamate. Other quenching methods involve adding 20-50mM Tris, lysine, glycine or ethanolamine; however, these primary amine-containing compounds modify carboxyls on protein #1. 7. Remove excess quenching reagent using a desalting column. Additional Information EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond and release of an isourea by-product (Figure 1). |
8樓2014-12-05 18:34:56
OD8811
至尊木蟲 (小有名氣)
- 應(yīng)助: 38 (小學(xué)生)
- 金幣: 17784.7
- 紅花: 1
- 帖子: 109
- 在線: 230.2小時(shí)
- 蟲號(hào): 1952119
- 注冊(cè): 2012-08-23
- 性別: GG
- 專業(yè): 高分子物理與高分子物理化
|
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, EDAC or EDCI) is a water soluble carbodiimide usually obtained as the hydrochloride. It is typically employed in the 4.0-6.0 pH range. It is generally used as a carboxyl activating agent for the coupling of primary amines to yield amide bonds. Additionally, EDC can also be used to activate phosphate groups in order to form phosphomono-esters and phosphodiesters. Common uses for this carbodiimide include peptide synthesis, protein crosslinking to nucleic acids, but also in the preparation of immunoconjugates. EDC is often used in combination with N-hydroxysuccinimide (NHS) for the immobilisation of large biomolecules. http://en.wikipedia.org/wiki/1-E ... opyl%29carbodiimide |
9樓2014-12-05 18:42:53
ayeee
新蟲 (初入文壇)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 567.9
- 帖子: 31
- 在線: 86.5小時(shí)
- 蟲號(hào): 2664623
- 注冊(cè): 2013-09-18
- 性別: MM
- 專業(yè): 中國(guó)古代文學(xué)
|
樓主你好，不好意思打擾你了，我最近也想制備不溶于水的聚丙烯酸納米纖維膜，能請(qǐng)問你是怎么制備的嗎？或者有什么參考文獻(xiàn)可以推薦一下嗎？真的非常感謝，期待你能回復(fù)我！ 發(fā)自小木蟲Android客戶端 |
10樓2017-03-10 22:57:08
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 junshane 的主題更新
| 10 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 303求調(diào)劑 +7 | DLkz1314. 2026-03-30 | 7/350 |
|
|---|---|---|---|---|
|
|
[考研] 300求調(diào)劑，材料科學(xué)英一數(shù)二 +18 | leaflight 2026-03-24 | 18/900 |
|
|
|
[考研]
 一志愿武漢理工，總分321，英一數(shù)二，求老師收留。
+11
|
nnnnnnn5 2026-03-25 | 11/550 |
|
|
|
[考研] 356求調(diào)劑 +4 | gysy?s?a 2026-03-28 | 4/200 |
|
|
|
[考研] 356求調(diào)劑 +3 | gysy?s?a 2026-03-28 | 3/150 |
|
|
|
[考研] 一志愿華理，數(shù)一英一285求A區(qū)調(diào)劑 +8 | AZMK 2026-03-25 | 12/600 |
|
|
[考研] 復(fù)試調(diào)劑 +3 | raojunqi0129 2026-03-28 | 3/150 |
|
|
|
[考研] 085701環(huán)境工程，267求調(diào)劑 +16 | minht 2026-03-26 | 16/800 |
|
|
|
[考研] 295求調(diào)劑 +5 | 1428151015 2026-03-27 | 6/300 |
|
|
|
[考研] 0703化學(xué)求調(diào)劑，各位老師看看我�。�！ +5 | 祁祺祺 2026-03-25 | 5/250 |
|
|
|
[考研] 265求調(diào)劑11408 +3 | 劉小鹿lu 2026-03-27 | 3/150 |
|
|
|
[碩博家園] 北京林業(yè)大學(xué)碩導(dǎo)招生廣告 +6 | kongweilin 2026-03-26 | 8/400 |
|
|
|
[考研] 機(jī)械學(xué)碩310分，數(shù)一英一，一志愿211本科雙非找調(diào)劑信息 +3 | @357 2026-03-25 | 3/150 |
|
|
|
[考研] 281求調(diào)劑 +6 | Koxui 2026-03-24 | 7/350 |
|
|
|
[考研] 290分調(diào)劑求助 +3 | 吉祥止止陳 2026-03-25 | 3/150 |
|
|
|
[考研] 0854人工智能方向招收調(diào)劑 +4 | 章小魚567 2026-03-24 | 4/200 |
|
|
|
[考研] 293求調(diào)劑 +7 | 加一一九 2026-03-24 | 7/350 |
|
|
|
[考研] 一志愿北化315 求調(diào)劑 +3 | akrrain 2026-03-24 | 3/150 |
|
|
|
[考研] 材料專碩找調(diào)劑 +5 | 哈哈哈吼吼吼哈 2026-03-23 | 5/250 |
|
|
|
[考研]
材料考研調(diào)劑生
+3
|
黃粱一夢(mèng)千年 2026-03-24 | 3/150 |
|