版塊導(dǎo)航: 正在加載中...

登錄注冊

應(yīng)《網(wǎng)絡(luò)安全法》要求，自2017年10月1日起，未進(jìn)行實名認(rèn)證將不得使用互聯(lián)網(wǎng)跟帖服務(wù)。為保障您的帳號能夠正常使用，請盡快對帳號進(jìn)行手機(jī)號驗證，感謝您的理解與支持！

24小時熱門版塊排行榜

返回列表

夕凝夕凝

金蟲 (小有名氣)

應(yīng)助: 3 (幼兒園)
金幣: 1447.9
散金: 18
紅花: 8
帖子: 205
在線: 58.4小時
蟲號: 2327281
注冊: 2013-03-07
性別: MM
專業(yè): 食品科學(xué)

[求助] 璇峰府蹇欐寚鍑鴻繖孌墊枃瀛楁秹?婂埌浜嗗摢浜涚數(shù)娉蟲妧鏈紵已有1人參與

請你指出這段文字涉???到了哪些電泳技術(shù)。（25分，答案采用中文書寫??????）??????電泳
SDS-PAGE was carried out as described by Laemmli on 12% polyacrylamide gels. For runs under nonreducing conditions, 2-mercaptoethanol was omitted in the denaturing buffer. Polypeptide bands were stained with Coomassie blue R-250. The Mr of the polypeptides was determined by comparison with a standard protein solution (GE Healthcare, Milan, Italy) containing phosphorylase b (94 kDa), bovine serum albumin (67 kDa), egg albumin (45 kDa), carbonic anhydrase (29 kDa), trypsin inhibitor (20 kDa), and lysozyme (14 kDa). Nonreducing/reducing double SDS-PAGE was performed as follows. After SDS-PAGE under nonreducing conditions, the lane of interest was excised with a razor blade and incubated in 3 mL of equilibration buffer (375 mM Tris-HCl, pH 8.8, 6 M urea, 2% SDS, 20% glycerol) with 65 mM DTT for 20 min and then with 245 mM iodoacetamide in the same buffer without DTT for 20 min. The gel lane was then placed horizontally on the top of a 15% polyacrylamide gel and sealed with 0.5% agarose, prepared in Laemmli running buffer. For two-dimensional IEF/SDS-PAGE, isoelectric focusing (IEF) was performed on 7 cm, pH 3-10 nonlinear IPG strips (GE Healthcare). The strips were rehydrated overnight in a solution containing 7 M urea, 2 M thiourea, 2% CHAPS, 65 mM DTT, and 2% IPG buffer pH 3-10 (GE Healthcare) containing 10 μg of the protein sample. Strips were focused at 8000 Vh, with a maximum of 2500 V, at 20 ℃ using a Multiphor II electrophoresis unit (GE Healthcare). Prior to the second dimension, strips were incubated in equilibration buffer with 65 mM DTT for 10 min and then with 245 mM iodoacetamide in the same buffer without DTT for 10 min.

[ ????????? http://www.gaoyang168.com/3g ]

回復(fù)此樓

» 猜你喜歡

0832食品調(diào)劑推薦已經(jīng)有0人回復(fù)
食品調(diào)劑-總分291-有中核論文導(dǎo)師一作、本人二作-一志愿南昌大學(xué)學(xué)碩-介紹詳細(xì) 已經(jīng)有12人回復(fù)
食品科學(xué)論文潤色/翻譯怎么收費? 已經(jīng)有215人回復(fù)
法國里爾大學(xué)_INRAE 2026招收食品方向CSC博士生已經(jīng)有0人回復(fù)
331求調(diào)劑，一志愿合工大食品工程（086003）已經(jīng)有2人回復(fù)
303分一志愿江南大學(xué)發(fā)酵工程學(xué)碩本科參加競賽有實驗室經(jīng)歷有酒廠質(zhì)檢員工作經(jīng)歷已經(jīng)有5人回復(fù)
331食品工程求調(diào)劑已經(jīng)有1人回復(fù)
一志愿福建農(nóng)林大學(xué) 09 食品與營養(yǎng) 初試323 已經(jīng)有0人回復(fù)
08600生物與醫(yī)藥調(diào)劑，其他方向也可以已經(jīng)有1人回復(fù)
鈣離子的存在形式已經(jīng)有0人回復(fù)

» 本主題相關(guān)價值貼推薦，對您同樣有幫助:

請高手幫忙分析一下這個PCR產(chǎn)物凝膠電泳圖已經(jīng)有5人回復(fù)
能否批量修改電泳膠圖？？已經(jīng)有10人回復(fù)
DGGE 垂直電泳灌膠技術(shù) 各位大俠幫幫忙已經(jīng)有13人回復(fù)
同工酶電泳圖片如何處理，請幫忙分析如下照片，謝謝已經(jīng)有9人回復(fù)
請大家?guī)兔纯次业牡鞍纂娪緢D，謝謝大家了已經(jīng)有10人回復(fù)
求大俠們幫忙翻譯一段文字（漢語翻譯成英語）已經(jīng)有5人回復(fù)
PCR電泳圖怎么會這樣？各位幫忙分析一下吧已經(jīng)有12人回復(fù)
【求助/交流】部分酶切的電泳圖不知為何成這個樣子？有經(jīng)驗的蟲蟲們幫忙解釋一下吧！已經(jīng)有10人回復(fù)
外文期刊中蛋白質(zhì)電泳圖（SDS-PAGE）如何處理已經(jīng)有4人回復(fù)
外文期刊要求圖和文字分開是什么意思已經(jīng)有8人回復(fù)

存在的就是合理的

1樓 2015-01-06 09:19:21

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

LS920706

銅蟲 (正式寫手)

高級勤雜工

應(yīng)助: 30 (小學(xué)生)
金幣: 198
散金: 50
紅花: 5
帖子: 360
在線: 113.1小時
蟲號: 1620552
注冊: 2012-02-16
性別: MM
專業(yè): 藥物學(xué)其他科學(xué)問題

【答案】應(yīng)助回帖

感謝參與，應(yīng)助指數(shù) +1

標(biāo)題亂碼，你這是求助什么？

贊一下

回復(fù)此樓

只有想不到，沒有做不到

2樓2015-01-06 11:56:06

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

相關(guān)版塊跳轉(zhuǎn) 我要訂閱樓主夕凝夕凝的主題更新