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Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy Cows Journal of Animal and Veterinary Advances |
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Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy Cows 作者:Liu, L (Liu, Li)[ 1 ] ; Yuan, ZR (Yuan, Zhengrong)[ 1 ] ; Chen, C (Chen, Cui)[ 1 ] ; Zhang, LM (Zhang, Limin)[ 1 ] ; Chen, XJ (Chen, Xiaojie)[ 1 ] ; Liu, XD (Liu, Xidong)[ 1 ] ; Gao, X (Gao, Xue)[ 1 ] ; Li, JY (Li, Junya)[ 1 ] ; Gao, HJ (Gao, Huijiang)[ 1 ] ; Zhang, LP (Zhang, Lupei)[ 1 ] 更多內(nèi)容 JOURNAL OF ANIMAL AND VETERINARY ADVANCES 卷: 11 期: 14 頁: 2588-2593 出版年: 2012 查看期刊信息 摘要 To study the inflammatory response of Peripheral Blood Mononuclear Cells (PBMCs) to actue phases of Staphylococcus aureus (S. aureus) mastitis in dairy cows using gene expression data, the mRNA expression levels of six genes (Iinterleukin (IL)-8, Differentiation (CD) 14, Macrophage Colony Stimulating Factor Receptor (M-CSFR), Toll-Like Receptor 2 (TLR2), Chemokine Receptor 2 (CCR2) and CX3C Chemokine Receptor 1 (CX(3)CR1) in Peripheral Blood Mononuclear Cells (PBMCs) in infected cows (n = 3) with S. aureus relative to uninfected controls (n = 31 were measured at 0 day before inoculation and 1-3 days post inoculation (dpi) by the real-time quantitative PCR. Results indicated the expression of IL-8, CD14 and CCR2 gene were exhibited significantly (p<0.05) up-regulated within 2 dpi while there was no significant difference in the expression of M-CSFR, 7LR2 and CX(3)CR1 genes at 1-3 dpi. Additionally, concentrations of plasma lipopolysaccharide Binding Protein (LBP) and Somatic Cell Counts (SCCs) were evaluated. Together these data reveal that the upregulation of gene expression is probably related to the recruitment and activation of PBMCs. 關(guān)鍵詞 作者關(guān)鍵詞 airy cow; mastitis; S. aureus; gene expression; PBMCs; ChinaKeyWords Plus:BOVINE MASTITIS; BINDING PROTEIN; HOLSTEIN COWS; MONOCYTES; EXPRESSION; SUBPOPULATIONS; INFECTION; RECEPTOR; CD14 作者信息 通訊作者地址: Xu, SZ (通訊作者) [顯示增強組織信息的名稱] Chinese Acad Agr Sci, Inst Anim Sci, Lab Mol Biol & Bovine Breeding, 2 Yuanmingyuan W Rd, Beijing 100193, Peoples R China. 地址: [顯示增強組織信息的名稱] [ 1 ] Chinese Acad Agr Sci, Inst Anim Sci, Lab Mol Biol & Bovine Breeding, Beijing 100193, Peoples R China 基金資助致謝 基金資助機構(gòu) 授權(quán)號 2011BAD28B04 2010jc-2 查看基金資助信息 出版商 MEDWELL ONLINE, ANSINET BUILDING, 308-LASANI TOWN, SARGODHA RD, FAISALABAD, 38090, PAKISTAN 類別 / 分類 研究方向:Veterinary Sciences Web of Science 類別:Veterinary Sciences 文獻信息 文獻類型:Article 語種:English 入藏號: WOS:000307808900035 ISSN: 1680-5593 其他信息 IDS 號: 992VS Web of Science 核心合集中的 "引用的參考文獻": 19 Web of Science 核心合集中的 "被引頻次": 0 |
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