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Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy Cows

Journal of Animal and Veterinary Advances

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1樓 2015-01-27 23:46:50

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liuli439

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3樓: Originally posted by carefu at 2015-01-28 08:52:40
WOS:000307808900035

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Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy Cows

作者:Liu, L (Liu, Li)[ 1 ] ; Yuan, ZR (Yuan, Zhengrong)[ 1 ] ; Chen, C (Chen, Cui)[ 1 ] ; Zhang, LM (Zhang, Limin)[ 1 ] ; Chen, XJ (Chen, Xiaojie)[ 1 ] ; Liu, XD (Liu, Xidong)[ 1 ] ; Gao, X (Gao, Xue)[ 1 ] ; Li, JY (Li, Junya)[ 1 ] ; Gao, HJ (Gao, Huijiang)[ 1 ] ; Zhang, LP (Zhang, Lupei)[ 1 ] 更多內(nèi)容

JOURNAL OF ANIMAL AND VETERINARY ADVANCES

卷: 11

期: 14

頁: 2588-2593

出版年: 2012

查看期刊信息
摘要

To study the inflammatory response of Peripheral Blood Mononuclear Cells (PBMCs) to actue phases of Staphylococcus aureus (S. aureus) mastitis in dairy cows using gene expression data, the mRNA expression levels of six genes (Iinterleukin (IL)-8, Differentiation (CD) 14, Macrophage Colony Stimulating Factor Receptor (M-CSFR), Toll-Like Receptor 2 (TLR2), Chemokine Receptor 2 (CCR2) and CX3C Chemokine Receptor 1 (CX(3)CR1) in Peripheral Blood Mononuclear Cells (PBMCs) in infected cows (n = 3) with S. aureus relative to uninfected controls (n = 31 were measured at 0 day before inoculation and 1-3 days post inoculation (dpi) by the real-time quantitative PCR. Results indicated the expression of IL-8, CD14 and CCR2 gene were exhibited significantly (p<0.05) up-regulated within 2 dpi while there was no significant difference in the expression of M-CSFR, 7LR2 and CX(3)CR1 genes at 1-3 dpi. Additionally, concentrations of plasma lipopolysaccharide Binding Protein (LBP) and Somatic Cell Counts (SCCs) were evaluated. Together these data reveal that the upregulation of gene expression is probably related to the recruitment and activation of PBMCs.
關(guān)鍵詞

作者關(guān)鍵詞

airy cow; mastitis; S. aureus; gene expression; PBMCs; China

KeyWords Plus:BOVINE MASTITIS; BINDING PROTEIN; HOLSTEIN COWS; MONOCYTES; EXPRESSION; SUBPOPULATIONS; INFECTION; RECEPTOR; CD14
作者信息

通訊作者地址: Xu, SZ (通訊作者)
[顯示增強組織信息的名稱] Chinese Acad Agr Sci, Inst Anim Sci, Lab Mol Biol & Bovine Breeding, 2 Yuanmingyuan W Rd, Beijing 100193, Peoples R China.

地址:
[顯示增強組織信息的名稱] [ 1 ] Chinese Acad Agr Sci, Inst Anim Sci, Lab Mol Biol & Bovine Breeding, Beijing 100193, Peoples R China

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基金資助機構(gòu) 授權(quán)號

2011BAD28B04

2010jc-2
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出版商

MEDWELL ONLINE, ANSINET BUILDING, 308-LASANI TOWN, SARGODHA RD, FAISALABAD, 38090, PAKISTAN
類別 / 分類

研究方向:Veterinary Sciences

Web of Science 類別:Veterinary Sciences
文獻信息

文獻類型:Article

語種:English

入藏號: WOS:000307808900035

ISSN: 1680-5593
其他信息

IDS 號: 992VS

Web of Science 核心合集中的 "引用的參考文獻": 19

Web of Science 核心合集中的 "被引頻次": 0

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5樓2015-01-28 12:22:27

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5樓: Originally posted by limh770522 at 2015-01-28 12:22:27 Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy Cows 作者:Liu, L (Liu, Li) ; Yuan, ZR (Yuan, Zhengrong) ; Chen, C (Chen, ...

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