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2011164112新蟲 (初入文壇)
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Whether 3-nitrotyrosine is important in normal cellular processes or is likely to affect specific biological pathways remains unclear. Using GPS-YNO2, a recently described 3-nitrotyrosine prediction algorithm, a set of predictions for nitrated residues in the human proteome was generated. In total, 9.27 per cent of the proteome was predicted to be nitratable (27 922/ 301 091). By matching the predictions against a set of curated and experimentally validated 3-nitrotyrosine sites in human proteins, it was found that GPS-YNO2 is able to predict 73.1 per cent (404/553) of these sites. Furthermore, of these sites, 42 have been shown to be nitrated endogenously, with 85.7 per cent (36/42) of these predicted to be nitrated. This demonstrates the feasibility of using the predicted dataset for a whole proteome analysis. |
至尊木蟲 (著名寫手)
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3-硝基酪氨酸是否對正常的細胞過程很重要或者有可能影響特定的生物學途徑,一切尚不明確。 通過使用 GPS-YNO2,生成了一個新的描述3-硝基酪氨酸的預測算法以及一組關(guān)于人類蛋白質(zhì)組中消化殘留物的語言。 總計,蛋白質(zhì)組9.27%的組分被預測具有可硝化性 (27 922/301 091)。 通過匹配預測及有針對性的監(jiān)管和實驗驗證3-硝基酪氨酸在人類蛋白質(zhì)中的位點,發(fā)現(xiàn)GPS-YNO2能預測73.1%(404/553)的位點。 此外,在那些位點中,42個已經(jīng)被證明了是內(nèi)生硝化,其中有85.7%(36/42) 被預測到能硝化。 這論證了使用預測數(shù)據(jù)庫來分析整個蛋白質(zhì)組的可行性。 |

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