版塊導(dǎo)航: 正在加載中...

登錄注冊

應(yīng)《網(wǎng)絡(luò)安全法》要求，自2017年10月1日起，未進(jìn)行實名認(rèn)證將不得使用互聯(lián)網(wǎng)跟帖服務(wù)。為保障您的帳號能夠正常使用，請盡快對帳號進(jìn)行手機(jī)號驗證，感謝您的理解與支持！

24小時熱門版塊排行榜

返回列表

Malyhon

新蟲 (小有名氣)

應(yīng)助: 0 (幼兒園)
金幣: 26.5
帖子: 56
在線: 13.9小時
蟲號: 3398024
注冊: 2014-09-04

[求助] 幾句論文求大神翻譯成中文謝謝

2.5. Acridine Orange Staining. At the fifth day of culturing, cells
grown on different filmswerewashedwith PBS and stained with 0.1mg/mL
acridine orange (Sigma-Aldrich) at room temperature in darkness for
30 min. Then, all the samples were washed with PBS to remove the residual
dye and observed through an inverted fluorescencemicroscope (Nikon Ti).
2.6. Cell Viability Assay. Cell viability was measured with Cell
Counting Kit-8 (CCK-8, Dojindo, Japan) according to the manufacture’s
protocol. The amount of the formazan dye generated by the activity of
dehydrogenases in cells on highly water-soluble tetrazolium salt is directly
proportional to the number of living cells. Cells were cultured on different
PHBVfilms in 48-well plates with six repeats. In brief, at 1, 3, and 5 days after
cell seeding, 20 μL ofCCK-8 solution was added to each well and incubated
in the incubator for 3 h; then, 100 μL of solution per well was transferred to
a 96-well plate and read at 450 nm with a microplate reader (Symergy HT,
Biotek). Cell viability can be continually tested in the same well without any
toxicity to the cells.

回復(fù)此樓

» 猜你喜歡

317求調(diào)劑已經(jīng)有6人回復(fù)
304求調(diào)劑已經(jīng)有4人回復(fù)
085700資源與環(huán)境308求調(diào)劑已經(jīng)有7人回復(fù)
265求調(diào)劑已經(jīng)有5人回復(fù)
279分求調(diào)劑一志愿211 已經(jīng)有3人回復(fù)
求調(diào)劑已經(jīng)有3人回復(fù)
一志愿吉林大學(xué)材料學(xué)碩321求調(diào)劑已經(jīng)有12人回復(fù)
286分人工智能專業(yè)請求調(diào)劑愿意跨考！已經(jīng)有3人回復(fù)
329求調(diào)劑已經(jīng)有5人回復(fù)
申請回稿延期一個月，編輯同意了。但系統(tǒng)上的時間沒變，給編輯又寫郵件了，沒回復(fù) 已經(jīng)有4人回復(fù)

1樓 2015-04-27 15:14:06

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

jiangguofeng

金蟲 (著名寫手)

翻譯EPI: 248
應(yīng)助: 41 (小學(xué)生)
金幣: 25886.4
紅花: 25
帖子: 2100
在線: 567.1小時
蟲號: 72410
注冊: 2005-06-04
專業(yè): 合成藥物化學(xué)

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
Malyhon: 金幣+11, ★★★★★最佳答案 2015-04-28 12:31:44

2.5.吖啶橙著色。
在培養(yǎng)的第五天,用PBS洗滌生長于不同膜上的細(xì)胞并在室溫下于暗處用0.1mg/mL的吖啶橙（西格瑪-奧德里奇產(chǎn)品）對其著色。然后,所有樣品用PBS洗滌以除去殘留的染料，并通過倒置熒光顯微鏡(尼康Ti)觀察。

2.6.細(xì)胞活力檢測。
細(xì)胞活力是用細(xì)胞計數(shù)試劑盒-8 (CCK-8,日本,同仁公司）按照生產(chǎn)商的方案測量的。由高水溶性四唑鹽上的細(xì)胞內(nèi)脫氫酶的活性度所產(chǎn)生的甲臜染料的量與活細(xì)胞數(shù)目成正比。細(xì)胞于48-孔板中不同PHBV膜上培養(yǎng)，重復(fù)六次。總之,在細(xì)胞接種后的第1、3和5天, 將20μL的CCK -8溶液加到每一孔中并于細(xì)菌培養(yǎng)器中培養(yǎng)３小時；然后,將每孔100μL溶液移至一個96 -孔板上并用酶標(biāo)儀（Symergy HT, 美國伯騰儀器有限公司）測出450 nm處的讀數(shù)。細(xì)胞活力檢測可以于同一個對細(xì)胞沒有任何毒性的孔中反復(fù)地進(jìn)行。

贊一下(1人)

回復(fù)此樓

2樓2015-04-27 20:22:01

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

相關(guān)版塊跳轉(zhuǎn) 我要訂閱樓主 Malyhon 的主題更新