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jiangyucai木蟲 (小有名氣)
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[求助]
英文中目的是什么?本人第一做蛋白,完全不懂,
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Crystal shikonin (8 mg) was mixed with the cell lysate of MCF-7 (2 mg protein), rotated at 41C for 2 h and centrifuged to remove the supernatant. The precipitate (crystal shikonin with bound cellular protein) was washed three times with chilled washing buffer (50 mMTris–HCl (pH 7.5), 150 mM NaCl, 2 mMEDTA, 10% glycerol, 1 mMNaF) by centrifugation (5000 r.p.m. for 1 min at 41C). The crystal shikonin with bound cellular protein was dissolved and boiled in SDS–PAGE sample buffer for 10 min. The samples were loaded and separated on 10% SDS–polyacrylamide gel and stained by silver staining. The bands were cut, digested in gel and loaded for LC-MS-MS spectrometer analysis by Shanghai Applied Protein Technology Co Ltd. Proteins were identified by searching the databases of Swiss-Prot using SEQUEST 希望給翻譯一下,第一話中旋轉(zhuǎn),需要什么儀器?第三句話中溶解有什么溶劑?,第四句中質(zhì)譜分析的目的是什么?能否提供幾篇這方面的文章,,感謝 [ Last edited by jiangyucai on 2015-7-5 at 18:19 ] |
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