 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 867 | 回復(fù): 4 | |||
[交流]
大家看看這段話怎么翻譯 已有2人參與
|
|
Suspension cultures were started with either 50ml or 100 ml SFM in a 1 liter spinner bottle. Half the medium was changed every 3-4 days by centrifuging the cells and adding them back with the fresh medium. Eventually, the volume of the medium was increased to 200 ml. Thereafter, half of the medium was changed three times a week by allowing the culture to remain stationary for 15-30 min, pipetting off the top half of the medium with a 100 ml pipette and then adding an equal volume of fresh medium. Very few cells were lost during this procedure because most of the cells grew in dense aggregates which settled out quickly. 翻譯其實還能翻的通，就是那個換液，然后體積增加最后增加到200ml，具體怎么操作過來的不是很明白 |
回復(fù)此樓» 猜你喜歡
346求調(diào)劑[0856]
已經(jīng)有7人回復(fù)
-
一志愿山大07化學(xué) 332分 四六級已過 本科山東雙非 求調(diào)劑！
已經(jīng)有3人回復(fù)
-
310求調(diào)劑
已經(jīng)有3人回復(fù)
-
機械專碩299求調(diào)劑至材料
已經(jīng)有4人回復(fù)
-
070300化學(xué)319求調(diào)劑
已經(jīng)有7人回復(fù)
-
08工科 320總分 求調(diào)劑
已經(jīng)有6人回復(fù)
-
一志愿天津大學(xué)化學(xué)工藝專業(yè)（081702）315分求調(diào)劑
已經(jīng)有12人回復(fù)
-
307求調(diào)劑
已經(jīng)有3人回復(fù)
-
265求調(diào)劑
已經(jīng)有3人回復(fù)
-
301求調(diào)劑
已經(jīng)有10人回復(fù)
» 本主題相關(guān)價值貼推薦，對您同樣有幫助:
- 請大家?guī)臀铱纯吹谒亩卧趺捶g，這兩句話是相互矛盾了嗎？ tks~
已經(jīng)有5人回復(fù)
- 英文好的進(jìn)來教教我，看看我這段翻譯的問題在哪里，謝謝啦！
已經(jīng)有9人回復(fù)
- 大家?guī)臀铱纯碿ritical job skills是什么意思
已經(jīng)有2人回復(fù)
- 12年考研英語85分，我也來談?wù)勛约旱膹?fù)習(xí)過程（原創(chuàng)）
已經(jīng)有80人回復(fù)
- 百度文庫里的，轉(zhuǎn)給大家看看，Python的學(xué)習(xí)
已經(jīng)有12人回復(fù)
- 極好的分析長難句的文章！以前收藏的，與大家分享一下，覺得好就頂��！
已經(jīng)有10人回復(fù)
- 論文要交出版費，大家?guī)臀铱纯聪旅孢@段話是什么意思？
已經(jīng)有4人回復(fù)
- 【轉(zhuǎn)載】考研高分密碼
已經(jīng)有91人回復(fù)
2樓2015-10-08 19:44:03
|
我也知道換液，關(guān)鍵是怎么換。Half the medium was changed every 3-4 days by centrifuging the cells and adding them back with the fresh medium. Eventually, the volume of the medium was increased to 200 ml. Thereafter, half of the medium was changed three times a week by allowing the culture to remain stationary for 15-30 min, pipetting off the top half of the medium with a 100 ml pipette and then adding an equal volume of fresh medium.按照這個說法，新鮮培養(yǎng)基替換一般原來的培養(yǎng)基，體積應(yīng)該是不增加的。 |
3樓2015-10-09 11:12:23
4樓2015-10-10 14:45:20
5樓2015-10-11 20:40:56
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 herway 的主題更新
| 5 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 346求調(diào)劑[0856] +4 | WayneLim327 2026-03-16 | 7/350 |
|
|---|---|---|---|---|
|
|
[考研] 083200學(xué)碩321分一志愿暨南大學(xué)求調(diào)劑 +3 | innocenceF 2026-03-17 | 3/150 |
|
|
|
[考研] 一志愿 西北大學(xué) ，070300化學(xué)學(xué)碩，總分287，雙非一本，求調(diào)劑。 +4 | 晨昏線與星海 2026-03-19 | 4/200 |
|
|
|
[考研]
 材料與化工 322求調(diào)劑
+4
|
然11 2026-03-19 | 4/200 |
|
|
|
[考研] 藥學(xué)383 求調(diào)劑 +3 | 藥學(xué)chy 2026-03-15 | 5/250 |
|
|
|
[考研] 本人考085602 化學(xué)工程 專碩 +19 | 不知道叫什么！ 2026-03-15 | 21/1050 |
|
|
|
[考研] 一志愿 南京航空航天大學(xué)大學(xué) ，080500材料科學(xué)與工程學(xué)碩 +5 | @taotao 2026-03-20 | 5/250 |
|
|
|
[考研] 求調(diào)劑 +3 | eation27 2026-03-20 | 3/150 |
|
|
|
[考研] 08工學(xué)調(diào)劑 +5 | 用戶573181 2026-03-20 | 5/250 |
|
|
|
[考研] 085410人工智能專碩317求調(diào)劑（0854都可以） +4 | xbxudjdn 2026-03-18 | 4/200 |
|
|
[考研] 266求調(diào)劑 +5 | 陽陽哇塞 2026-03-14 | 10/500 |
|
|
|
[考研] 286求調(diào)劑 +6 | lemonzzn 2026-03-16 | 10/500 |
|
|
|
[考研] 344求調(diào)劑 +6 | knight344 2026-03-16 | 7/350 |
|
|
|
[考研] 311求調(diào)劑 +6 | 26研0 2026-03-15 | 6/300 |
|
|
|
[考研] 326求調(diào)劑 +5 | 上岸的小葡 2026-03-15 | 6/300 |
|
|
|
[考研]
11408 一志愿西電，277分求調(diào)劑
+3
|
zhouzhen654 2026-03-16 | 3/150 |
|
|
|
[考研] 中科院材料273求調(diào)劑 +4 | yzydy 2026-03-15 | 4/200 |
|
|
|
[考研] 277材料科學(xué)與工程080500求調(diào)劑 +3 | 自由煎餅果子 2026-03-16 | 3/150 |
|
|
|
[考研] 0703化學(xué)調(diào)劑 290分有科研經(jīng)歷，論文在投 +7 | 膩膩gk 2026-03-14 | 7/350 |
|
|
|
[考研] 0856求調(diào)劑 +3 | 劉夢微 2026-03-15 | 3/150 |
|