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大家看看這段話怎么翻譯 已有2人參與
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Suspension cultures were started with either 50ml or 100 ml SFM in a 1 liter spinner bottle. Half the medium was changed every 3-4 days by centrifuging the cells and adding them back with the fresh medium. Eventually, the volume of the medium was increased to 200 ml. Thereafter, half of the medium was changed three times a week by allowing the culture to remain stationary for 15-30 min, pipetting off the top half of the medium with a 100 ml pipette and then adding an equal volume of fresh medium. Very few cells were lost during this procedure because most of the cells grew in dense aggregates which settled out quickly. 翻譯其實還能翻的通，就是那個換液，然后體積增加最后增加到200ml，具體怎么操作過來的不是很明白 |
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2樓2015-10-08 19:44:03
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我也知道換液，關鍵是怎么換。Half the medium was changed every 3-4 days by centrifuging the cells and adding them back with the fresh medium. Eventually, the volume of the medium was increased to 200 ml. Thereafter, half of the medium was changed three times a week by allowing the culture to remain stationary for 15-30 min, pipetting off the top half of the medium with a 100 ml pipette and then adding an equal volume of fresh medium.按照這個說法，新鮮培養(yǎng)基替換一般原來的培養(yǎng)基，體積應該是不增加的。 |
3樓2015-10-09 11:12:23
4樓2015-10-10 14:45:20
5樓2015-10-11 20:40:56
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