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| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
中國的_BOY木蟲 (著名寫手)
我是瞎說的
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[求助]
求翻譯這段話
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Frozen stocks of the bacterial isolates were obtained from the Cornell University Food Safety Laboratory (CU-FSL) isolate collection. Cultures were streaked from −80°C frozen stock onto brain heart infusion (Difco, Sparks, MD) agar and incubated for 18 h at 32°C. A colony from each strain was used to inoculate 5 mL of sterile brain heart infusion broth in 16-mm glass test tubes, which were then incubated for 48 h with aeration (shaking at 220 rpm) at 32°C. A 1-mL aliquot of the culture was then centrifuged at 14,000 × gand the supernatant was removed. The resulting cell pellet was resuspended in 1 mL of phosphate dilution buffer (Weber Scientific, Trenton, NJ), diluted to 1,000 to 10,000 cfu/mL, and inoculated into commercially purchased UHT pasteurized milk (1% milk; Organic Valley, La Farge, WI) for an approximate inoculum of 2 to 3 log cfu/mL. Inocula were enumerated by spiral plating the appropriate dilution on SPC agar (EMD, Gibbstown, NJ), followed by incubation at 32°C for 24 to 48 h. Inoculated UHT milk was incubated at 6°C for 48 h to simulate refrigerated storage conditions. After a 48-h incubation at 6°C, appropriate dilutions of inoculated UHT samples prepared in phosphate dilution buffer were plated in duplicate on (1) SPC pour plates (SPCP); (2) SPC plates inoculated by spiral plating (SPCS); (3) CVTA [plate count agar (EMD, Gibbstown, NJ) with 2,3,5-triphenyltetrazolium chloride (Sigma-Aldrich, St. Louis, MO)] pour plates (CVTAP); (4) CVTA plates inoculated by spiral plating (CVTAS); (5) VRBA plates inoculated by pour plating (Difco, Sparks, MD); (6) VRBA with an overlay of approximately 5 mL of VRBA agar (VRBAO); (7) CPETRI (3M); or (8) MAC (Difco) inoculated by spiral plating. All media were incubated at 32°C for 48 h, with enumeration after 24 and 48 h. Three biological replications of the experiment were completed. 題目：評(píng)估一下可用于檢測(cè)巴氏殺菌乳中假單胞菌屬種類的各種選擇性培養(yǎng)基 |
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中國的_BOY
木蟲 (著名寫手)
我是瞎說的
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2樓2015-10-22 07:52:05
中國的_BOY
木蟲 (著名寫手)
我是瞎說的
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歡迎前來捧場(chǎng) 3樓2015-10-22 13:26:59
baoshanqiu
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【答案】應(yīng)助回帖
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中國的_BOY: 金幣+100, 翻譯EPI+1, ★★★★★最佳答案, 謝謝啊，翻譯的不錯(cuò) 2015-10-23 09:01:57
中國的_BOY: 金幣+100, 翻譯EPI+1, ★★★★★最佳答案, 謝謝啊，翻譯的不錯(cuò) 2015-10-23 09:01:57
| 細(xì)菌分離物的凍存原種來自于康奈爾大學(xué)食品安全實(shí)驗(yàn)室(CU-FSL) 菌種保存。將培養(yǎng)物自−80°C凍存原種劃線接種于腦心浸液(Difco, Sparks, MD)瓊脂上32°C培養(yǎng)18 h。每一株選一個(gè)菌落接種于無菌16-mm玻璃試管內(nèi)的5ml腦心浸液肉湯，置 32°C通氣（220 rpm振搖）培養(yǎng)48h。取每份1ml培養(yǎng)物于14,000 × g離心、去上清。所得細(xì)胞沉淀物用1ml磷酸鹽稀釋緩沖液(Weber Scientific, Trenton, NJ)再懸浮，稀釋的 1,000 至10,000 cfu/mL,以大約2 to 3 log cfu/mL量接種于市售超高溫巴氏消毒的牛奶(1% 牛奶; Organic Valley, La Farge, WI)。培養(yǎng)菌液計(jì)數(shù)以適當(dāng)稀釋度于SPC（標(biāo)準(zhǔn)平板計(jì)數(shù)）瓊脂 (EMD, Gibbstown, NJ)上做旋轉(zhuǎn)平板涂布，32°C 培養(yǎng) 24至 48 h 。接種了細(xì)菌的超高溫消毒牛奶置6°C 孵育48 h模擬冰箱貯存條件。接種了細(xì)菌的超高溫消毒牛奶樣品經(jīng)6°C 孵育48 h后，用磷酸鹽稀釋緩沖液適當(dāng)稀釋，雙份接種于（1） SPC傾注平板 (SPCP); （2）旋轉(zhuǎn)平板涂布接種SPC (SPCS); (3)CVTA（結(jié)晶紫四氮唑） [平板計(jì)數(shù)瓊脂(EMD, Gibbstown, NJ) 含 2,3,5-氯化三苯四氮唑 (Sigma-Aldrich, St. Louis, MO)] 傾注平板(CVTAP); (4) 旋轉(zhuǎn)平板涂布接種CVTA(CVTAS); (5)VRBA（紫紅膽汁瓊脂）傾注接種平板 (Difco, Sparks, MD); (6) 以大約5mlVRBA覆蓋VRBA瓊脂 (VRBAO); (7)CPETRI (大腸桿菌皿膜） (3M);或(8) 旋轉(zhuǎn)平板涂布接種MAC（麥康凱）(Difco)。所有培養(yǎng)基置32°C 培養(yǎng)48 h，于24和48h后計(jì)數(shù)。實(shí)驗(yàn)進(jìn)行了3次生物學(xué)重復(fù)。 |
4樓2015-10-22 23:12:59
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