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| 本帖產(chǎn)生 1 個 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
中國的_BOY木蟲 (著名寫手)
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Frozen stocks of the bacterial isolates were obtained from the Cornell University Food Safety Laboratory (CU-FSL) isolate collection. Cultures were streaked from −80°C frozen stock onto brain heart infusion (Difco, Sparks, MD) agar and incubated for 18 h at 32°C. A colony from each strain was used to inoculate 5 mL of sterile brain heart infusion broth in 16-mm glass test tubes, which were then incubated for 48 h with aeration (shaking at 220 rpm) at 32°C. A 1-mL aliquot of the culture was then centrifuged at 14,000 × gand the supernatant was removed. The resulting cell pellet was resuspended in 1 mL of phosphate dilution buffer (Weber Scientific, Trenton, NJ), diluted to 1,000 to 10,000 cfu/mL, and inoculated into commercially purchased UHT pasteurized milk (1% milk; Organic Valley, La Farge, WI) for an approximate inoculum of 2 to 3 log cfu/mL. Inocula were enumerated by spiral plating the appropriate dilution on SPC agar (EMD, Gibbstown, NJ), followed by incubation at 32°C for 24 to 48 h. Inoculated UHT milk was incubated at 6°C for 48 h to simulate refrigerated storage conditions. After a 48-h incubation at 6°C, appropriate dilutions of inoculated UHT samples prepared in phosphate dilution buffer were plated in duplicate on (1) SPC pour plates (SPCP); (2) SPC plates inoculated by spiral plating (SPCS); (3) CVTA [plate count agar (EMD, Gibbstown, NJ) with 2,3,5-triphenyltetrazolium chloride (Sigma-Aldrich, St. Louis, MO)] pour plates (CVTAP); (4) CVTA plates inoculated by spiral plating (CVTAS); (5) VRBA plates inoculated by pour plating (Difco, Sparks, MD); (6) VRBA with an overlay of approximately 5 mL of VRBA agar (VRBAO); (7) CPETRI (3M); or (8) MAC (Difco) inoculated by spiral plating. All media were incubated at 32°C for 48 h, with enumeration after 24 and 48 h. Three biological replications of the experiment were completed. 題目：評估一下可用于檢測巴氏殺菌乳中假單胞菌屬種類的各種選擇性培養(yǎng)基 |
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中國的_BOY
木蟲 (著名寫手)
我是瞎說的
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2樓2015-10-22 07:52:05
中國的_BOY
木蟲 (著名寫手)
我是瞎說的
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歡迎前來捧場 3樓2015-10-22 13:26:59
baoshanqiu
至尊木蟲 (著名寫手)
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中國的_BOY: 金幣+100, 翻譯EPI+1, ★★★★★最佳答案, 謝謝啊，翻譯的不錯 2015-10-23 09:01:57
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| 細(xì)菌分離物的凍存原種來自于康奈爾大學(xué)食品安全實驗室(CU-FSL) 菌種保存。將培養(yǎng)物自−80°C凍存原種劃線接種于腦心浸液(Difco, Sparks, MD)瓊脂上32°C培養(yǎng)18 h。每一株選一個菌落接種于無菌16-mm玻璃試管內(nèi)的5ml腦心浸液肉湯，置 32°C通氣（220 rpm振搖）培養(yǎng)48h。取每份1ml培養(yǎng)物于14,000 × g離心、去上清。所得細(xì)胞沉淀物用1ml磷酸鹽稀釋緩沖液(Weber Scientific, Trenton, NJ)再懸浮，稀釋的 1,000 至10,000 cfu/mL,以大約2 to 3 log cfu/mL量接種于市售超高溫巴氏消毒的牛奶(1% 牛奶; Organic Valley, La Farge, WI)。培養(yǎng)菌液計數(shù)以適當(dāng)稀釋度于SPC（標(biāo)準(zhǔn)平板計數(shù)）瓊脂 (EMD, Gibbstown, NJ)上做旋轉(zhuǎn)平板涂布，32°C 培養(yǎng) 24至 48 h 。接種了細(xì)菌的超高溫消毒牛奶置6°C 孵育48 h模擬冰箱貯存條件。接種了細(xì)菌的超高溫消毒牛奶樣品經(jīng)6°C 孵育48 h后，用磷酸鹽稀釋緩沖液適當(dāng)稀釋，雙份接種于（1） SPC傾注平板 (SPCP); （2）旋轉(zhuǎn)平板涂布接種SPC (SPCS); (3)CVTA（結(jié)晶紫四氮唑） [平板計數(shù)瓊脂(EMD, Gibbstown, NJ) 含 2,3,5-氯化三苯四氮唑 (Sigma-Aldrich, St. Louis, MO)] 傾注平板(CVTAP); (4) 旋轉(zhuǎn)平板涂布接種CVTA(CVTAS); (5)VRBA（紫紅膽汁瓊脂）傾注接種平板 (Difco, Sparks, MD); (6) 以大約5mlVRBA覆蓋VRBA瓊脂 (VRBAO); (7)CPETRI (大腸桿菌皿膜） (3M);或(8) 旋轉(zhuǎn)平板涂布接種MAC（麥康凱）(Difco)。所有培養(yǎng)基置32°C 培養(yǎng)48 h，于24和48h后計數(shù)。實驗進(jìn)行了3次生物學(xué)重復(fù)。 |
4樓2015-10-22 23:12:59
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