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中國的_BOY木蟲 (著名寫手)
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| After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998). Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21°C, our findings suggest that incubating plates at 32°C can accurately detect a diversity of Pseudomonas isolates in milk. |
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捐助貴賓 (著名寫手)
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中國的_BOY: 金幣+30, ★★★★★最佳答案, 辛苦了 2015-10-30 14:06:03
中國的_BOY: 金幣+30, ★★★★★最佳答案, 辛苦了 2015-10-30 14:06:03
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After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. 經(jīng)過48小時的潛伏期，可以觀察到對于某些選擇性培養(yǎng)基中，尤其是CVTA培養(yǎng)基中，探測效率有所提高。 因此48小時的潛伏期導(dǎo)致了，在探測假單胞菌時，總體效率的提升 in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998). 對于牛奶培養(yǎng)基， 它與CPETRI 和CVTA培養(yǎng)基一樣，推薦進(jìn)行48小時的潛伏期 （Frank 和 Yousef2004的研究成果），而與MAC培養(yǎng)基不一樣，后者是推薦進(jìn)行18-24小時的潛伏期(Difco 1998年的研究成果)。 Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. 我們的研究結(jié)果標(biāo)明，在CVTA培養(yǎng)基中板式乳樣和進(jìn)行板式培養(yǎng)48小時，是探測巴氏殺菌奶中假單胞菌污染的最佳方法。 These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). 這些研究結(jié)果，支持運(yùn)用標(biāo)準(zhǔn)方法檢測乳制品中，列舉牛奶培養(yǎng)基中革蘭氏陰性的有機(jī)體的方法。 However, whereas Standard Methods recommends incubating plates at 21°C, our findings suggest that incubating plates at 32°C can accurately detect a diversity of Pseudomonas isolates in milk.。 然而，標(biāo)準(zhǔn)方法建議在21攝氏度下進(jìn)行板式培養(yǎng)，而我們的研究結(jié)果建議在32攝氏度下進(jìn)行板式培養(yǎng)，后者能準(zhǔn)確探測出牛奶培養(yǎng)基中各種假單胞菌獨(dú)立菌落。 |
2樓2015-10-29 21:52:14
baoshanqiu
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中國的_BOY: 金幣+37, 翻譯EPI+1, ★★★★★最佳答案, 謝謝了。 2015-10-30 14:05:47
中國的_BOY: 金幣+37, 翻譯EPI+1, ★★★★★最佳答案, 謝謝了。 2015-10-30 14:05:47
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After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998). 48-h培養(yǎng)后，一些選擇性培養(yǎng)基，特別是CVTA的檢測效率有所提高。因此48-h培養(yǎng)整體改善了牛奶中假單胞菌屬的檢測，這與推薦的CPETRI 和 CVTA (Frank 和 Yousef, 2004)培養(yǎng)48-h一致，但與推薦的MAC培養(yǎng)時間不一致，后者為 18 到 24 h (Difco, 1998)。 |
3樓2015-10-30 00:49:49
baoshanqiu
至尊木蟲 (著名寫手)
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Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21°C, our findings suggest that incubating plates at 32°C can accurately detect a diversity of Pseudomonas isolates in milk. 我們的結(jié)果表明在CVTA上接種牛奶樣品并將平皿培養(yǎng) 48 h是檢測巴氏消毒牛奶中假單胞菌污染的最佳方法。這些發(fā)現(xiàn)與乳制品檢查的標(biāo)準(zhǔn)方法(Frank 和 Yousef, 2004)中出現(xiàn)的牛奶中革蘭氏陰性菌計(jì)數(shù)法相一致。然而，標(biāo)準(zhǔn)法推薦在21°C培養(yǎng)平皿，我們發(fā)現(xiàn)在32°C培養(yǎng)平皿能準(zhǔn)確檢出牛奶中各種假單胞菌分離物。 |
4樓2015-10-30 01:05:31
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