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[求助]
求組文獻句子翻譯
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看文獻有一句話不太懂。 The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme. 這句話怎么翻譯好一些呢？ 共價結合法傾向于酶特定的方向？？感覺不太對啊�。� |
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genhunter
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袁小小然: 金幣+3 2015-11-19 23:05:24
愛與雨下: 金幣+1 2015-11-20 07:09:39
袁小小然: 金幣+3 2015-11-19 23:05:24
愛與雨下: 金幣+1 2015-11-20 07:09:39
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The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme. 用EDC/NHS方法很到的增幅最少(增加2.8倍), 這可以用共價交聯趨于將酶以特定的走向排布來解釋。 |
2樓2015-11-19 14:01:30
genhunter
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3樓2015-11-19 14:34:23
lz3333
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袁小小然: 金幣+5 2015-11-19 23:05:37
愛與雨下: 金幣+1 2015-11-20 07:09:55
袁小小然: 金幣+5 2015-11-19 23:05:37
愛與雨下: 金幣+1 2015-11-20 07:09:55
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The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme. 是固定化酶的文獻么？我畢業(yè)論文做的固定化酶。 翻譯需要看上下文的意思，才可以更好的翻譯出來，不過就字面的意思應該翻譯為： EDC/NHS法增加的最少，僅2.8倍，共價連接法可以將酶定向（固定化）這一事實可解釋這一現象。 |
» 本帖已獲得的紅花（最新10朵）
袁小小然4樓2015-11-19 15:15:20
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Each sample was a square piece of Whatman no 1 paper with a1 × 1 cm sample area defined with wax (XEROX Phaser printer) [10].Samples for each immobilization method were coded and mea-sured in random order. A 10 L of solution was needed to wet thepaper completely, if not stated otherwise this volume was used forall modifications. All solutions, if not stated differently were pre-pared in PBS pH 5.5. In case of layer-by-layer methods paper wascovered with 3 bilayers starting with anionic polymer. In the secondand third bilayer cationic polymer was mixed with Glucose oxidaseto obtain final concentration of 2.5 mg/mL (92.5 U/mL) of enzyme.In the case of subsequent methods: EDC, EDC/NHS, entrapment inammonium alginate and all encapsulation methods several batcheswith different concentration of reagents and reaction times wereprepared. Table 1 lists only the optimized conditions of aforemen-tioned methods. In case of encapsulation methods, smaller capsuleswere dispersed on sample papers with a pipette, larger capsuleswere transported manually on paper and left to dry. |
5樓2015-11-19 23:03:45
6樓2015-11-19 23:05:07
lz3333
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【答案】應助回帖
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Each sample was a square piece of Whatman no 1 paper with a 1 × 1 cm sample area defined with wax (XEROX Phaser printer) [10]. 每個樣品都是在whatman 1號方形紙上面的一個1 × 1 cm的樣品區(qū)域，使用蠟進行固定。 Samples for each immobilization method were coded and measured in random order. 每種固定方法的樣品都是隨機編號和測量。 A 10 L of solution was needed to wet the paper completely, if not stated otherwise this volume was used for all modifications. 如未標記，所有修飾方法均使用10L溶液來完全潤濕樣品紙片。 All solutions, if not stated differently were prepared in PBS pH 5.5. 所有溶液，如未特殊標記，均在PBS，pH5.5條件下制備。 In case of layer-by-layer methods paper was covered with 3 bilayers starting with anionic polymer. 層疊法中紙片需用3層膜覆蓋，第一層是陰離子聚合物， In the second and third bilayer cationic polymer was mixed with Glucose oxidase to obtain final concentration of 2.5 mg/mL (92.5 U/mL) of enzyme. 第二層和第三層的陽離子聚合物需要與葡糖氧化酶混合，以達到最終酶濃度為2.5 mg/mL (92.5 U/mL) 你黏貼過來的文獻，斷句有問題，你看看原文吧，下面是我自己根據意思翻譯的。 In the case of subsequent methods: EDC, EDC/NHS, entrapment in ammonium alginate and all encapsulation methods，several batches with different concentration of reagents and reaction times were prepared. 接下來的方法，EDC、 EDC/NHS法中，使用海藻酸銨進行截留；所有封包法中使用不同的試劑濃度和反應時間分別進行了制備。 Table 1 lists only the optimized conditions of a fore-mentioned methods. In case of encapsulation methods, smaller capsules were dispersed on sample papers with a pipette, larger capsules were transported manually on paper and left to dry. 表1僅列舉了前面提到方法的最佳條件。在封包法中，小囊使用移液管分散在樣品紙上，大囊手動轉移到紙上并晾干。 |
7樓2015-11-20 09:51:03
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