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[求助]
幫我看看這兩句話的翻譯有沒(méi)有問(wèn)題
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細(xì)胞加入Trizol后吹打,室溫靜置5Min,直接提取RNA或放入-80℃冰箱凍存。待Trizol融化后,加入200ul氯仿/ml Trizol,顛倒混勻(30s),室溫放置3Min。 Add Trizol to cells and homogenize the mixture, stand for 5 minutes for extracting RNA at room temperature or store RNA in -80 degrees Celsius refrigerator.Once Tizol melted, add 200ul chloroform/ml Trizol and homogenize(30s),stand for 3 minutes at room temperature,centrifuge at 12,000g for 15 min. |
至尊木蟲 (知名作家)
Translator and Proofreader
至尊木蟲 (知名作家)
Translator and Proofreader
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這不是抽提RNA嗎,你中文敘述的也太復(fù)雜了。英文部分用詞不準(zhǔn)確:例如混勻和homogenize不是一回事。另外,你的英文寫的和你所讀過(guò)的英文文獻(xiàn)應(yīng)該是有很大出入的。我給你總結(jié)一下吧。把你自己寫的忘了: Trizol was added to the cells, cell lysates were mixed, stood for 5 min at room temperature and used for RNA extraction immediately or stored at -80C. In the latter case, cell lysates were thawed completely and 200 ul of chloroform/ml Trizol were added. The mixture was mixed thoroughly for 30 s, stood for 3 min at room temperature, and centrifuged for 15 min at 12,000 x g. |
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