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| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進(jìn)行查看 | ||
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[求助]
幫我看看這兩句話的翻譯有沒有問題
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細(xì)胞加入Trizol后吹打，室溫靜置5Min，直接提取RNA或放入-80℃冰箱凍存。待Trizol融化后，加入200ul氯仿/ml Trizol，顛倒混勻（30s），室溫放置3Min。 Add Trizol to cells and homogenize the mixture, stand for 5 minutes for extracting RNA at room temperature or store RNA in -80 degrees Celsius refrigerator.Once Tizol melted, add 200ul chloroform/ml Trizol and homogenize(30s),stand for 3 minutes at room temperature,centrifuge at 12,000g for 15 min. |
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3樓2015-12-06 09:46:18
2樓2015-12-05 22:51:45
ssssllllnnnn 
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【答案】應(yīng)助回帖
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這不是抽提RNA嗎，你中文敘述的也太復(fù)雜了。英文部分用詞不準(zhǔn)確：例如混勻和homogenize不是一回事。另外，你的英文寫的和你所讀過的英文文獻(xiàn)應(yīng)該是有很大出入的。我給你總結(jié)一下吧。把你自己寫的忘了： Trizol was added to the cells, cell lysates were mixed, stood for 5 min at room temperature and used for RNA extraction immediately or stored at -80C. In the latter case, cell lysates were thawed completely and 200 ul of chloroform/ml Trizol were added. The mixture was mixed thoroughly for 30 s, stood for 3 min at room temperature, and centrifuged for 15 min at 12,000 x g. |
4樓2015-12-06 10:10:40
5樓2015-12-06 10:58:41
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