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| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
[求助]
幫我看看這兩句話(huà)的翻譯有沒(méi)有問(wèn)題
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細(xì)胞加入Trizol后吹打，室溫靜置5Min，直接提取RNA或放入-80℃冰箱凍存。待Trizol融化后，加入200ul氯仿/ml Trizol，顛倒混勻（30s），室溫放置3Min。 Add Trizol to cells and homogenize the mixture, stand for 5 minutes for extracting RNA at room temperature or store RNA in -80 degrees Celsius refrigerator.Once Tizol melted, add 200ul chloroform/ml Trizol and homogenize(30s),stand for 3 minutes at room temperature,centrifuge at 12,000g for 15 min. |
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2樓2015-12-05 22:51:45
3樓2015-12-06 09:46:18
ssssllllnnnn 
至尊木蟲(chóng) (知名作家)
Translator and Proofreader
- 翻譯EPI: 1690
- 應(yīng)助: 452 (碩士)
- 金幣: 31580.9
- 紅花: 100
- 帖子: 7681
- 在線(xiàn): 19966.6小時(shí)
- 蟲(chóng)號(hào): 3328089
- 注冊(cè): 2014-07-17
- 專(zhuān)業(yè): 腫瘤發(fā)生
【答案】應(yīng)助回帖
|
這不是抽提RNA嗎，你中文敘述的也太復(fù)雜了。英文部分用詞不準(zhǔn)確：例如混勻和homogenize不是一回事。另外，你的英文寫(xiě)的和你所讀過(guò)的英文文獻(xiàn)應(yīng)該是有很大出入的。我給你總結(jié)一下吧。把你自己寫(xiě)的忘了： Trizol was added to the cells, cell lysates were mixed, stood for 5 min at room temperature and used for RNA extraction immediately or stored at -80C. In the latter case, cell lysates were thawed completely and 200 ul of chloroform/ml Trizol were added. The mixture was mixed thoroughly for 30 s, stood for 3 min at room temperature, and centrifuged for 15 min at 12,000 x g. |
4樓2015-12-06 10:10:40
5樓2015-12-06 10:58:41
ssssllllnnnn
至尊木蟲(chóng) (知名作家)
Translator and Proofreader
- 翻譯EPI: 1690
- 應(yīng)助: 452 (碩士)
- 金幣: 31580.9
- 紅花: 100
- 帖子: 7681
- 在線(xiàn): 19966.6小時(shí)
- 蟲(chóng)號(hào): 3328089
- 注冊(cè): 2014-07-17
- 專(zhuān)業(yè): 腫瘤發(fā)生
6樓2015-12-06 11:06:35
7樓2015-12-06 11:10:21
ssssllllnnnn
至尊木蟲(chóng) (知名作家)
Translator and Proofreader
- 翻譯EPI: 1690
- 應(yīng)助: 452 (碩士)
- 金幣: 31580.9
- 紅花: 100
- 帖子: 7681
- 在線(xiàn): 19966.6小時(shí)
- 蟲(chóng)號(hào): 3328089
- 注冊(cè): 2014-07-17
- 專(zhuān)業(yè): 腫瘤發(fā)生
【答案】應(yīng)助回帖
★ ★ ★ ★ ★
drug.S: 金幣+5, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝。 2015-12-06 12:12:02
drug.S: 金幣+5, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝。 2015-12-06 12:12:02
|
OK Add Trizol to cells and lysate the cells by mixing the mixture thoroughly; stand the mixture for 5 minutes and then isolate RNA immediately at room temperature or store the lysates at -80C until further analysis. For stored samples, thaw the lysates completely and add 200 ul of chloroform/ml Trizol. Mix the mixture completely by inversion repeatedly for 30s and stand for 3 minutes at room temperature, followed by centrifugation at 12,000 x g for 15 min. |
8樓2015-12-06 11:45:29
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