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| 【獎勵】 本帖被評價67次,作者taozhikai增加金幣 52.6 個 | |||||||
[資源]
JACS最新超分子酶納米顆粒生物傳感器
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| ABSTRACT: Rapid and sensitive detection of pathogens is a key requirement for both environmental and clinicalsettings. We report here a colorimetric enzymenanoparticleconjugate system for detection of microbial contamination.In this approach, cationic gold nanoparticles (NPs)featuring quaternary amine headgroups are electrostaticallybound to an enzyme [β-galactosidase (β-Gal)], inhibitingenzyme activity. Analyte bacteria bind to the NP, whichreleases the β-Gal and restores its activity, providing anenzyme-amplified colorimetric readout of the binding event.Using this strategy, we have been able to quantify bacteria at con- centrations of 1 102 bacteria/mL in solution and 1 104 bacteria/mL in a field-friendly test strip format.[ Last edited by taozhikai on 2011-7-10 at 22:48 ] |
納米材料及生物材料前沿資源 | 蛋白質(zhì)生物學(xué)實驗經(jīng)驗 | JACS專題 | 生物材料 |
結(jié)構(gòu)生物學(xué) |





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