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sephadex G-25柱 蛋白質(zhì)分離 已有7人參與
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小弟現(xiàn)在在做有關(guān)過sephadex-25柱的實驗,望各位前輩不吝賜教關(guān)于如何做sephadex -25的柱子,以及運用 PS 小弟做的蛋白質(zhì)質(zhì)量在66000左右 |


鐵桿木蟲 (著名寫手)

木蟲 (著名寫手)
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Gel filtration chromatography separates proteins in order of large to small molecules. In general, large proteins will not enter the gel pores and will elute rapidly from the column in the void volume. Smaller proteins will repeatedly enter and leave the pores of the gel and therefore will remain longer in the column. Hence, proteins will elute in order of decreasing molecular size. Commercially Available Gel Filtration (GF) Gel Matrix Gel Type = Fractionation Range (molecular weight) Sephadex G-10 = <700 Sephadex G-25 = 1,000-5,000 Bio-Gel P-60 = 3,000-60,000 Sephadex G-75 = 3,000-70,000 Sephadex G-100 = 4,000-100,000 Bio-Gel P-100 = 5,000-100,000 Sephadex G-200 = 5,000-250,000 Bio-Gel P-200 = 30,000-200,000 Sephacryl S-300 = 10,000-1,500,000 Sepharose 2B = 70,000-40,000,000 |
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