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373668567
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[求助]
求一段英譯漢,在線翻譯器的請繞道哦
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First, 293 cells stably expressing Moloney gag and pol under control of cytomegalovirus (CMV) promoter-enhancer were transiently transfected by CaPO4 precipitation with 20 ug of pLXSN retroviral vector and 5 ug of the plasmid pMDG containing the vesicular stomatitis virus (VSVg) envelope downstream of the CMV promoter-enhancer. and a 100 ul sample was loaded into disposable chambers and centrifuged for 1 min onto glass slides in a cytocentrifuge。Staining was verified by visualization under a light microscope, and the slides were mounted with glass cover slips with the use of Permount mounting media 。The population of cells was categorized as either viable, apoptotic, or necrotic when analyzed by staining morphology. Counting was performed blind, meaning that each slide was given a number and the identity of each sample was not known during counting. The counting procedure made use of a light microscope at a magnification that allowed the incorporation of about 100 cells per field. Five fields were counted per slide, and each experimental condition was performed two times. |
金蟲 (著名寫手)
金蟲 (正式寫手)
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首先,293細胞中穩(wěn)定表達莫洛尼GAG和POL下控制巨細胞病毒(CMV)啟動子增強瞬時轉染CaPO4降水20微克的逆轉錄病毒載體PLXSN和5質粒含有水泡性口炎病毒(VSVg)PMDG UG信封下游CMV啟動子增強。 銷售100微升樣品裝入一次性商會cytocentrifuge在1分鐘到玻片離心染色光鏡下可視化驗證,并安裝與使用Permount安裝媒體的玻璃蓋玻片的幻燈片。。細胞的人口被歸類為 無論是可行的,細胞凋亡或壞死時,染色形態(tài)分析。點票是盲目的,意味著每張幻燈片被賦予了每個樣本的數量和身份,沒有在計數。點票程序在使用光學顯微鏡的放大,使約100個細胞每場納入。五個領域分別計數每張幻燈片,每個實驗條件下進行兩次。 |
木蟲 (小有名氣)
版主 (文壇精英)
米米
版主 (文壇精英)
米米
版主 (文壇精英)
米米
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首先,在巨細胞病毒(CMV)啟動子的增強子控制下,293個穩(wěn)定表達莫羅尼氏病毒“GAG和POL”(這個我不知道是啥)的細胞,被磷酸鈣沉淀、20微克的逆轉錄病毒載體PLXSN、5微克含有水泡性口炎病毒(VSVg) pMDG質?焖俎D染。 將100微升樣品裝入一次性容器內。在細胞離心機內離心1分鐘后放到玻片上。在光學顯微鏡下直接驗證染色,然后使用Permount安裝媒介將玻片和蓋玻片結合。根據染色后的形態(tài),細胞的群體被分類為能生存的,凋亡的,或者壞死的。直接計數將是隨機,意味著對于每塊玻片,數值和類別在計數過程中不知道的。計數過程將在光學顯微鏡下進行,視野中約有100個細胞。每塊玻片取五個可視區(qū)域,每種實驗條件下進行兩次計數。 有一些術語查不到,應該差不多了 |
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