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373668567
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[求助]
求一段英譯漢，在線翻譯器的請繞道哦
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First, 293 cells stably expressing Moloney gag and pol under control of cytomegalovirus (CMV) promoter-enhancer were transiently transfected by CaPO4 precipitation with 20 ug of pLXSN retroviral vector and 5 ug of the plasmid pMDG containing the vesicular stomatitis virus (VSVg) envelope downstream of the CMV promoter-enhancer. and a 100 ul sample was loaded into disposable chambers and centrifuged for 1 min onto glass slides in a cytocentrifuge。Staining was verified by visualization under a light microscope, and the slides were mounted with glass cover slips with the use of Permount mounting media 。The population of cells was categorized as either viable, apoptotic, or necrotic when analyzed by staining morphology. Counting was performed blind, meaning that each slide was given a number and the identity of each sample was not known during counting. The counting procedure made use of a light microscope at a magnification that allowed the incorporation of about 100 cells per field. Five fields were counted per slide, and each experimental condition was performed two times. |
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fjtony163
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★ ★ ★ ★ ★
373668567(金幣+1, 翻譯EPI+1): 不帶這么玩的哈，在線翻譯！ 2011-10-06 23:18:22
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sltmac: 2011-10-07 08:24:20
373668567(金幣+1, 翻譯EPI+1): 不帶這么玩的哈，在線翻譯！ 2011-10-06 23:18:22
sltmac(金幣-5, 翻譯EPI-1): 機(jī)器翻譯，EPI撤銷，并扣5BB以示警戒~~~ 2011-10-07 08:24:06
sltmac: 2011-10-07 08:24:20
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首先，293細(xì)胞中穩(wěn)定表達(dá)莫洛尼GAG和POL下控制巨細(xì)胞病毒（CMV）啟動子增強(qiáng)瞬時轉(zhuǎn)染CaPO4降水20微克的逆轉(zhuǎn)錄病毒載體PLXSN和5質(zhì)粒含有水泡性口炎病毒（VSVg）PMDG UG信封下游CMV啟動子增強(qiáng)。 銷售100微升樣品裝入一次性商會cytocentrifuge在1分鐘到玻片離心染色光鏡下可視化驗(yàn)證，并安裝與使用Permount安裝媒體的玻璃蓋玻片的幻燈片。。細(xì)胞的人口被歸類為 無論是可行的，細(xì)胞凋亡或壞死時，染色形態(tài)分析。點(diǎn)票是盲目的，意味著每張幻燈片被賦予了每個樣本的數(shù)量和身份，沒有在計(jì)數(shù)。點(diǎn)票程序在使用光學(xué)顯微鏡的放大，使約100個細(xì)胞每場納入。五個領(lǐng)域分別計(jì)數(shù)每張幻燈片，每個實(shí)驗(yàn)條件下進(jìn)行兩次。 |
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