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lixingliang新蟲 (小有名氣)
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[求助]
緊急求助！如何回答reviewer重做實驗的建議
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時逢圣誕期間，以為老外休假，沒想到今天收到主編回復，要求大修（所投雜志是Pesticide Biochemistry and Physiology ），和導師討論后意見出現(xiàn)分歧，不知道該怎么辦，向大家求助。 求助問題1：有兩個reviewer回復意見，都提出語言需要改進，老板很堅決的說不會花錢找公司改文章，頂多找個native speaker免費修改，這是老板的決定，本人沒辦法說服花錢找公司改。 求助問題2：我的研究是尋找一種藥物的作用靶點，其中一個reviewer提出藥物處理濃度太高，以至于結果不可信，建議用LD50的40% and 80%的濃度重復試驗。也就是說需要所有的實驗重新做，老板也很堅決的說了，實驗不會重復，也不會補數(shù)據(jù)，因為處理的藥物和處理對象都沒有了，不可能再做了。這可怎么辦��？如果不按照reviewer的建議以低濃度藥物重復試驗，會不會直接被拒？？ 以上是主要的問題，其他的都是很小的問題。先粘貼editor回復如下： Dear Dr. ###, In regard to your manuscript ###," we now have the reviewer's comments. Based on these comments, we see that your manuscript needs Major revision. I would appreciate it if you could address these points in your revision. Please consider the reviewer's comments very carefully in revising your manuscript and prepare a detailed "point-by-point" explanation of your revision/rebuttals. You are not required to follow every suggestion, but please provide your reasons for each rebuttal. For your guidance, reviewers' comments are appended below. Thank you for the opportunity to review your manuscript, and we look forward to receiving your revision. John Marshall Clark, PhD Pesticide Biochemistry and Physiology Reviewers' comments: Reviewer #1: This manuscript presents interesting results and provides useful methods for molluscicidal mechanism of A on Oncomelania hupensis. However, in my knowledge, the concentrations of A and Nic exposed to snails are too high to obtain the reliable results. The concentrations of 40% and 80% LD50 are much better for the study of molluscicidal mechanism. If possible, the authors should use the proper concentrations to repeat this study. The paper requires a revision of the language and grammar which should be done with the assistance of someone who is more conversant with the language. Reviewer #2: The authors investigated the effect of A on snail mitochondria. The molluscicidal activity of Ais already well established. The present investigations now provide some additional data on the possible mode of action. Generally, the paper is well prepared, the methods applied are appropriate and the conclusions drawn are conclusive.However, there are a number of points which need careful consideration before publication can be recommended: * The paper needs a very comprehensive revision with respect to English grammar and syntax. * Please write ginkgolic acids and niclosamide with a small initial letter throughout the manuscript * The investigations cited in reference [14] were conducted with LLC-MK(2) as well as HaCaT cells. * Page 7, line 1-2: Schistosomiasis joponica should be changed to S. japonica * Page 9: As described in "Statistical Analysis" correlation coefficients were calculated. However, no such results are given. * Page 9: Section 3.1 could entirely be deleted as most of these information are already given in the Materials and Methods section. * Page 10, line 4: LD50 values at 12 h are also shown in Tab. 2 * Page 10, Tab. 2: What statistical test was applied for the calculation of p-values? The legend to Tab. 2 refers to superscripts which do not occur in the Table. * Page 11, line 3: According to Fig. 4 the expression of adenylate kinase does already decrease at 12 h while the mRNA level for cytochrome b seems to drop down only after 24 and 48 h. This disagreement needs to be solved. * Page 11, line 16-18. Unpublished work should not be cited. * Page 12, line 13: Expression of five mitochondrial enzymes was allegedly investigated. ? * Page 13: Citation of reference 10 is incomplete. 我們實驗室的人都不容易畢業(yè)，原因嗎，這個大家都懂得  這篇文章能否發(fā)表對我意義很大，希望大家能伸出援助之手，幫幫我想想應對之策。祝大家圣誕快樂，元旦快樂��！ PS 本人金幣攢了很長時間，還是不多，先發(fā)一點，論文順利的話，全部發(fā)完，在所不惜��！ |
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lixingliang
新蟲 (小有名氣)
- 應助: 12 (小學生)
- 金幣: 383.5
- 散金: 100
- 帖子: 181
- 在線: 41.6小時
- 蟲號: 1254305
- 注冊: 2011-04-03
- 性別: GG
- 專業(yè): 生物化學
2樓2011-12-28 23:13:28
lixingliang
新蟲 (小有名氣)
- 應助: 12 (小學生)
- 金幣: 383.5
- 散金: 100
- 帖子: 181
- 在線: 41.6小時
- 蟲號: 1254305
- 注冊: 2011-04-03
- 性別: GG
- 專業(yè): 生物化學
3樓2011-12-29 12:33:21
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