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[求助]
金納米簇 Em=400nm
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以蛋白質(zhì)為模板,320nm激發(fā),400nm有發(fā)射,500nm激發(fā),660nm有發(fā)射,但是400nm處的強(qiáng)度比660nm強(qiáng)度高10倍左右 用365nm手提紫外燈肉眼看不出熒光 僅蛋白質(zhì)、金在相同的條件下都做了空白試驗(yàn),均無400及660nm發(fā)射光 請(qǐng)問這是納米簇的熒光嗎? |
專家顧問 (著名寫手)
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專家經(jīng)驗(yàn): +19 |
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In some papers, protein-templated AuNCs also exhibited emission peaks at around 450 nm, which was considered from the proteins. For example, see Nanoscale,2010, 2, 2769-2776 ( http://pubs.rsc.org/en/content/articlelanding/2010/nr/c0nr00377h) Since the intensity of the 400 nm band is very strong, it is better you rule out the possibility of Raman scattering from water. Change the excitation wavelength and see whether these bands keep unchanged... |
專家顧問 (著名寫手)
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專家經(jīng)驗(yàn): +19 |
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Then that emission band is possibly from the generated smaller-sized AuNCs (for exampe, Au8). This means the AuNCs you obtained contain different sized particles. Try to adjust your synthesis conditions, e.g., control the pH, then possibly you can obtain less/sharp emission bands. |
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之前有一鍋反應(yīng) 400nm處的發(fā)射波長不隨ex變化,但是后來重復(fù)試驗(yàn),發(fā)下幾乎所有的400nm處的發(fā)射都隨Ex變化;我試了光加蛋白質(zhì)及堿不加Au的空白試驗(yàn),Em也隨EX變化,但是強(qiáng)度遠(yuǎn)遠(yuǎn)沒有加Au時(shí)的強(qiáng),而且Em的峰位也不同。 請(qǐng)問我這個(gè)會(huì)是什么東西呢?會(huì)不會(huì)是水的Em的移動(dòng),導(dǎo)致峰位疊加,最后導(dǎo)致了我峰位的移動(dòng)呢? 感謝牛人指點(diǎn)。 |
銅蟲 (初入文壇)
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