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[求助]
金納米簇 Em=400nm
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以蛋白質為模板,320nm激發(fā),400nm有發(fā)射,500nm激發(fā),660nm有發(fā)射,但是400nm處的強度比660nm強度高10倍左右 用365nm手提紫外燈肉眼看不出熒光 僅蛋白質、金在相同的條件下都做了空白試驗,均無400及660nm發(fā)射光 請問這是納米簇的熒光嗎? |
專家顧問 (著名寫手)
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專家經(jīng)驗: +19 |
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In some papers, protein-templated AuNCs also exhibited emission peaks at around 450 nm, which was considered from the proteins. For example, see Nanoscale,2010, 2, 2769-2776 ( http://pubs.rsc.org/en/content/articlelanding/2010/nr/c0nr00377h) Since the intensity of the 400 nm band is very strong, it is better you rule out the possibility of Raman scattering from water. Change the excitation wavelength and see whether these bands keep unchanged... |
專家顧問 (著名寫手)
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專家經(jīng)驗: +19 |
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Then that emission band is possibly from the generated smaller-sized AuNCs (for exampe, Au8). This means the AuNCs you obtained contain different sized particles. Try to adjust your synthesis conditions, e.g., control the pH, then possibly you can obtain less/sharp emission bands. |
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