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我是驢金蟲 (小有名氣)
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[求助]
細(xì)菌全基因組提取時(shí)需要加入RNA酶嗎?
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| 請(qǐng)問大家,在提取混合體系細(xì)菌全基因組時(shí)需要加入RNA酶嗎? |


金蟲 (小有名氣)

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銅蟲 (小有名氣)
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不用加,有一快速提取法,供參考! 1. Add 100 μl of Solution A to the tail clip (2mm) 2. Place in heat block (preheated to 95°C) for 60 minutes 3. Add 100 μl of Solution B 4. Vortex briefly 5. Centrifuge at 2000rpm for 10 minutes 6. Remove 100 μl of supernatant and store in new labeled tube at 4°C * 7. Use 1μl per 25 μl PCR reaction * Optional-1 μl of supernatant can be removed directly from samples and added to pcr reaction. Solution A: 25mM NaOH/0.2mM EDTA Solution B: 40mM Tris-HCl Solutions A & B should be made up fresh each week, can be stored 1 week at 4°C STOCK SOLUTIONS: 1. 5M NaOH 2. 0.5M EDTA, pH 8.0 3. 1M Tris-HCl pH 8.0 |
新蟲 (初入文壇)
鐵桿木蟲 (著名寫手)
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